Journal of Applied Science Issue (5) September (2020) 62 A COMPARISON OF FLUORESCENCE AND ULTRAVIOLET DETECTORS USING THE RP–HPLC TECHNIQUE FOR THE DETERMINATION OF TRAMADOL HYDROCHLORIDE (TRM-HCl): A CASE STUDY OF AL-BAYDA CITY, LIBYA Noreldin S. Y. Abdolla 1 , Suad K. Omar 2* , Ibrahim H. Habib 3 , and Hana S. Mohamed 4 1 Chemistry Department, Faculty of Science, Tobrouk University, Tobrouk, Libya and the current address: Chemistry Department, University of Leicester, UK, 11Corfield Rise, LE3 1QF 2,4 Chemistry Department, Faculty of Science, Omar Al- Mukhtar University, Al- Bayda, Libya 3 National Research Centre, Applied Organic Chem. Dept., Dokki, Cairo, Egypt * suad.khalifa@omu.edu.ly Abstract A fast and sensitive chromatographic RP–HPLC technique for examining tramadol hydrochloride (TRM-HCl) in tablets through using fluorescence detection (FL-D) and ultraviolet detection (UV-D) is reported in this paper. The separation was carried out using the reverse phase method on a Brownlee BIO C18 analytical column with a mobile phase consisting of 0.1% acetic acid and acetonitrile (2.5:7.5 v/v), which was pumped with an isocratic elution at a flow rate of 1 ml/min. The LOD and LQD values obtained in the current study indicate that FL-D is more sensitive, and hence preferable to UV detectors in the quantification of TRM-HCl tablets over the entire concentration range used (5-125 μg/ml). The study showed that the mean percentage recoveries from five samples were 99.93- 100.023% (FL-D), which is somewhat similar to that of the UV-D (99.93-100.028). In conclusion, although Fl-D is acceptable for the quantification of tramadol tablets, UV-D offers higher detection sensitivity and reproducibility, particularly within concentrations that are low in the deposit collectors. Keywords: Tramadol; RP-HPLC; UV-D; FL-D; Sensitivity. Introduction Tramadol hydrochloride (TRM-HCl), (1R,2R) ‐2‐ ((dimethylamino) methyl) ‐1‐ (3 methoxyphenyl) cyclohexan‐1‐ol (Scheme1), is a central analgesic with a low affinity for opioid receptors. It is used to alleviate moderate to severe pain (Dayer, Collart and Desmeules, 1994; Kanaan et al., 2006; Abdel‐Megied and Badr El‐din, 2019; AlSamarrai, Abdoon and Hashim, 2019). TRM-HCl is a synthetic opioid agonist and serotonin reuptake inhibitor, whilst TRM is a safe drug for respiratory depression, cardiovascular and neurotransmission, unlike some other opioids (Klotz, 2003). This drug has two enantiomers and has a general