Downloaded from www.microbiologyresearch.org by IP: 54.70.40.11 On: Thu, 13 Dec 2018 03:15:43 Streptosporangium algeriense sp. nov., an actinobacterium isolated from desert soil Dalila Boubetra, 1 Noureddine Bouras, 1,2 Abdelghani Zitouni, 1 Peter Schumann, 3 Cathrin Spro ¨ er, 3 Nasserdine Sabaou 1 and Hans-Peter Klenk 4 Correspondence Nasserdine Sabaou sabaou@yahoo.fr Hans-Peter Klenk hans-peter.klenk@ncl.ac.uk 1 Laboratoire de Biologie des Syste ` mes Microbiens (LBSM), Ecole Normale Supe ´ rieure de Kouba, Alger, Algeria 2 De ´ partement de Biologie, Faculte ´ des Sciences de la Nature et de la Vie et Sciences de la Terre, Universite ´ de Ghardai ¨ a, BP 455, Ghardai ¨ a 47000, Algeria 3 DSMZ – German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, Germany 4 School of Biology, Newcastle University, Ridley Building, Newcastle upon Tyne, NE1 7RU, UK The taxonomic position of a novel actinobacterium, strain 169 T , isolated from a sample of Algerian Saharan soil, was determined using a polyphasic taxonomic approach. The aerial mycelium produced non-motile, round- to oval-shaped spores, with a smooth surface, which were sessile or carried by short sporophores. Chemotaxonomically, isolate 169 T showed the same results as members of the genus Streptosporangium, but madurose, the so far diagnostic sugar of the genus, was not detected in the whole-cell hydrolysate. Despite the absence of sporangia, the 16S rRNA gene sequence analysis confirmed that strain 169 T was a member of the genus Streptosporangium. Strain 169 T was most closely related to Streptosporangium jomthongense NBRC 110047 T (99.3 % 16S rRNA gene sequence similarity), which is the only non-sporangia-forming species reported among the genus. However, DNA–DNA hybridization studies with this species showed 60 % relatedness. Based upon genotypic and phenotypic data, a novel species, Streptosporangium algeriense sp. nov., is proposed, with 169 T (5DSM 45455 T 5MTCC 11561 T 5CCUG 62974 T ) as the type strain. Streptosporangium, the type genus of the family Streptospor- angiaceae, was proposed by Couch (1955) for the actino- bacteria producing aerial mycelium with spherical sporangia that enclose non-motile sporangiospores. These sporangia are produced by 21 species of the genus Streptos- porangium. However Intra et al. (2014) described a novel species of this genus with single roundish, smooth- surfaced-spores, which were formed through the inter- mediary of very short sporophores on aerial hyphae. This novel species, unlike the other members of the genus Strep- tosporangium, does not produce sporangia. Chemotaxono- mically, the genus Streptosporangium is characterized by the presence of meso-diaminopimelic acid in the cell wall and madurose in the whole-cell hydrolysates, di- and tetra- hydrogenated menaquinones with nine isoprene units, phosphatidylethanolamine and phospholipids containing glucosamine as diagnostic phospholipids, and complex mix- tures of iso-, anteiso-, saturated, unsaturated and 10-methyl- branched fatty acids (Quintana & Goodfellow, 2012). During taxonomic and antibiotic studies of Saharan acti- nobacteria, strain 169 T was isolated from a Saharan soil sample collected from Adrar palm grove in southern Algeria (latitude 278 539 N, longitude 08 169 E, altitude 262 m) by a dilution agar plating method using chitin– vitamin agar medium (Hayakawa & Nonomura, 1987) sup- plemented with actidione (50 mg ml 21 ). The presence of members of the genus Streptosporangium in these soils has already been reported (Boudjella et al., 2006). The aim of this study was to identify strain 169 T , which represents a novel species within the genus Streptosporan- gium, by using phenotypic, genotypic and phylogenetic approaches. Cultural characteristics were investigated on media from the International Streptomyces Project (ISP) (Shirling & Gottlieb, 1966), nutrient agar and Bennett’s agar (Waksman, 1961). The cultural characteristics were recorded after 7, 14 and 21 days of incubation at 30 8C. The colours of substrate and The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain 169 T is KP974644. Two supplementary figures and one supplementary table are available with the online Supplementary Material. International Journal of Systematic and Evolutionary Microbiology (2016), 66, 1034–1038 DOI 10.1099/ijsem.0.000829 000829 G 2015 IUMS Printed in Great Britain 1034