~ 1015 ~ Journal of Entomology and Zoology Studies 2021; 9(2): 1015-1018 E-ISSN: 2320-7078 P-ISSN: 2349-6800 www.entomoljournal.com JEZS 2021; 9(2): 1015-1018 © 2021 JEZS Received: 10-01-2021 Accepted: 12-02-2021 Ninan Jacob Professor and Head, Department of Veterinary Physiology, Rajiv Gandhi Institute of Veterinary Ducation and Research (RIVER), Kurumbapet, Pondicherry, India Padodara RJ Assistant Professor, Department of Veterinary Physiology and Biochemistry, College of Veterinary Science, J.A.U, Junagadh, Gujarat, India Arya JS Dean, Arawalli Veterinary College, Sikar, Rajasthan, India Gajbhiye PU Research Scientist (Retd.), Cattle Breeding Farm, J.A.U, Junagadh, Gujarat, India Corresponding Author: Ninan Jacob Professor and Head, Department of Veterinary Physiology, Rajiv Gandhi Institute of Veterinary Ducation and Research RIVER), Kurumbapet, Pondicherry, India WBC differential in Gir and Jaffarabadi animals at different physiological stages Ninan Jacob, Padodara RJ, Arya JS and Gajbhiye PU Abstract A study was conducted to establish the leucogram in Gir cattle and Jaffarabadi buffaloes at different age’s viz.1 wk, 1m, 3m, 6m, 12m, 24m and 36m, in lactating (1m, 2m, 3m) and non-lactating (pregnant and non-pregnant) and in castrated males and bulls. Thin blood smears were made and stained with Eosin and Methylene Blue (Field Stain) and the different types of leucocytes (DLC) were enumerated. The percentage of neutrophils in the blood smear is more than the percentage of lymphocytes at 1 wk age, indicating the pre-ruminant status of the animal. However, from one month of age onwards it was observed that the percentage of lymphocytes was more than the percentage of neutrophils as seen in ruminant animal. Keywords: Gir cattle, Jaffarabadi buffalo, lactation, pregnant, WBC 1. Introduction Haematological values help to assess the general health status of the individual animal and also the herd. The large number of species and breeds of domestic animals all over the country makes it is necessary to establish the normal baseline values for these breeds. Haematological values vary according to the species, breed, age, nutrition, physiological status, stress and climate. Leucocytes play an important role in the immune defense of the animal and make the infectious agent inactive by firstly destroying the bacteria or virus by phagocytosis and secondly by producing sensitised antibodies. As they can be transported quickly to the area of infection, they provide a very fast and strong defense against infectious agents. 2. Materials and Methods The study was carried out on male and female Gir cattle and Jaffarabadi buffaloes of various ages and physiological stages maintained under standard feeding and management conditions at the Cattle Breeding Farm, Junagadh Agricultural University, Junagadh, Gujarat and the haematological evaluation was carried out at the Department of Physiology and Biochemistry, College of Veterinary Science and Animal Husbandry, Junagadh Agricultural University, Junagadh, Gujarat. The project was approved by the Institutional Animal Ethics Committee (IAEC). Fresh whole blood (1ml) collected aseptically from the jugular vein of Gir cattle and Jaffarabadi buffaloes at different age’s (1 wk, 1, 3, 6, 12, 24 and 36 months) and physiological stages (lactating (1,2 and 3 months) and non-lactating (pregnant and non-pregnant), castrated males and bulls) were immediately subjected to haematological analyses. Thin blood smears were made and stained with Eosin and Methylene Blue (Field Stain) and the different types of leucocytes (DLC) were enumerated [1] . The data obtained were subjected to statistical analyses by using the Completely Randomised Design (CRD) [2] . 3. Results and Discussion The Mean ± S.E. differential leucocyte count (DLC) at different physiological stages for male and female Gir cattle and Jaffarabadi buffaloes are given in Table -1 and 2. 3.1 Lymphocytes (%): The lymphocyte count was significantly (P<0.05) lower at 1 wk age in both the sexes of both the species studied. The values increased significantly ( P<0.05) at 1 m age and increased as age advanced. The counts at 36 m in Gir and Jaffarabadi females and that at 12 m age in Gir and Jaffarabadi males was significantly (P<0.05) higher than the values at 1 wk age.