controls on EDTA pre-coated tubes. Demographic data were also collected by a questionnaire which was designed specifically for this study. Results: Our results showed that serum level of IP-10 was significantly higher in the patients than healthy controls (P<0.001). We also showed that relapsing remitting multiple sclerosis (RRMS) patients with a good economic status had elevated IP-10 (CXCL10) compared to median group (P<0.01). The circulating level of IP-10 (CXCL10) was significantly increased in female compared to male RRMS patients (P<0.01) and in patients who received less than 3 years treatment (P < 0.01). Conclusion: Based on the results of this study, it can probably be concluded that serum level of IP-10 have an important role in pathogenesis of multiple sclerosis. It is also worth noting that these factors could probably use as pivotal biological markers in diagnosis and possible treatment factors. Keywords: Multiple sclerosis, IP-10 (CXCL10), Polymorphism, Chemokine doi:10.1016/j.clinbiochem.2011.08.680 Poster – [A-10-253-1] The study of introns 22 and 1 inversions of the F8 gene in severe hemophilia A (HA) patients by inverse shifting polymerase chain reaction (IS-PCR) Narges Roozafzay, Leila Kokabee, Hoorfar Morteza Karimipoor Pasteur Avenue, Tehran, Iran E-mail addresses: n_roozafza@yahoo.com (N. Roozafzay), lkokabee@yahoo.com (L. Kokabee), mortezakarimi@yahoo.com (H.M. Karimipoor) Introduction: Hemophilia A is a bleeding disorder caused by different mutations in F8 gene while the most common mutation is inversion. The inversion of intron 22 has been detected by long distance PCR (LD-PCR) or Southern blot. Recently IS-PCR method has been introduced. The aim of this study was to detect the inversion of introns 1 and 22 by IS-PCR in severe HA patients. Methods: Inversion of Introns 22 and 1 was assessed by IS-PCR in 16 severe HA patients from Isfahan. After obtaining the consent from them, genomic DNA was extracted from peripheral blood leukocytes. IS-PCR includes treatment of genomic DNA by BclI restriction enzyme, followed by self-ligation to create cyclic DNA and finally PCR analysis. Results: In order to improve the molecular diagnosis of Inv22 and Inv1 of F8 gene we applied a novel IS-RCR diagnostic method. We assessed 16 severe HA patients with this technique. Five patients showed Inv22 type1 and one out of four was diagnosed as Inv1. Conclusion: Recombination between intron 22h-1 and its inversely oriented copies, intron 22h-2 and intron22h-3, caused inversions of intron 22 type 2 and type 1, respectively. We found five Inv22 type1 cases and one Inv1 in 16 HA patients that confirms Inv22 type1 is more frequent than Inv22 type2. IS-PCR is a time-saving method that improved the molecular diagnosis of HA and evaluation of carriers and HA patients could be used in prenatal diagnosis. This genotyping system may be adapted to all known rearrangements in the human genome. Keywords: Hemophilia A, Inversion, Inverse shifting-PCR doi:10.1016/j.clinbiochem.2011.08.681 Poster – [A-10-260-1] Association of FAS (-607 A/G) and FAS Ligand (-844 C/T) gene polymorphisms with breast cancer in Zahedan, Southeast Iran Aliakbar Fazaeli, Mohammad Hashemi, Farshid Arbabi, Ebrahim Eskandari, Mohsen Taheri Zahedan University of Medical Sciences, Zahedan, Iran E-mail addresses: ali_fazaeli@zaums.ac.ir (A. Fazaeli), mhd.hashemi@gmail.com (M. Hashemi), farshidarbabi@gmail.com (F. Arbabi), eenasab@yahoo.com (E. Eskandari), aafhbz@gmail.com (M. Taheri) Introduction: FAS and FAS Ligand (FASL) system plays a key role in apoptotic signaling via extrinsic pathway. It has been proposed that down- regulation of this pathway may facilitate formation of tumor cells. The aim of the present study was to examine the association of promoter polymorphism of FAS (-607 A/G) and FASL (-844 C/T) with breast cancer risk. Methods: This case–control study was done on 75 breast cancer patients and 152 population-based normal women. Genomic DNA was extracted from whole blood samples. We inspected polymorphisms in FAS (-607 A/G) and FASL (-844 C/T) by using tetra ARMS-PCR method. Results: The results showed that there was significant difference between case and control groups regarding FAS (-607 A/G) and FASL (-844 C/T) polymorphisms that might be risk factors for breast cancer in our population. Keywords: Breast cancer, Fas, FasL doi:10.1016/j.clinbiochem.2011.08.682 Poster – [A-10-271-1] Computer-based model within E2 conserved region of hepatitis C virus Pooneh Mokarram, Zohreh Mostafavi-Pour, Younes Hosseini, Reza Hajizadeh Mohammad Shiraz University of Medical Sciences, Shiraz, Iran E-mail address: mokaram2@gmail.com (P. Mokarram) Introduction: Spermatogenesis, a fundamental process in male reproductive system, requires a series of tightly controlled epigenetic and genetic events in germ cells. JHDM2A; JmjC-domain-containing histon demethylase 2A also known as JHDM1A; is essential for spermatogenesis and is the key epigenetic regulator expressed in testis. This enzyme is specifically demethylates mono and dimethy- lated histon H3 lysin 9. JHDM2A directly bounds to the core promoter regions of transition nuclear protein 1(Tnp1) and protamine1 ( Prm1). Products of these genes are required for packaging and condensation of sperm chromatin. Human Jhdm2a is located on 2p11.2 and has 27 exones. Exon 26 of this gene has 135 base pair long. Material and methods: 150 infertile men that belonged to azoospermia and oligozoospermia groups were selected and their blood samples were obtained for DNA extraction. Genomic DNA from these samples and 50 normal fertile men was used for PCR with specific primer (primer designed with oligo6 software). After PCR in appropriate conditions, the samples were examined using single strand conformation polymorphism (SSCP) for screening any muta- tion that might exist in exon 26. Results and conclusion: This study is under progress. We anticipate that point mutations in this region may correlate with men infertility and sperm abnormality. Keywords: E2, HCV, Computer-based designing doi:10.1016/j.clinbiochem.2011.08.683 Abstracts S276