http://www.revistadechimie.ro REV.CHIM.(Bucharest)♦70♦ No. 7 ♦2019 2608 Two Adhesive Systems-effect on Adhesion to Tooth Structure LARISA SIMONA DEAC 1 , KAMEL EARAR 2 *, ADELA LOREDANA COLCERIU BURTEA 1 *, ALEXANDRA STEFANIA BERGHE 1 , AURORA ANTONIAC 3 , ADA GABRIELA DELEAN 1 , VICENTIU SACELEANU 4 , ANCA DANIELA RAICIU 5 *, IOANA MARIA HODISAN 1 1 Iuliu Hatieganu University of Medicine and Pharmacy, Faculty of Dental Medicine, 8 Victor Babes Str., 400012, Cluj-Napoca, Romania 2 Dunarea de Jos University of Galati, Medicine and Pharmacy Faculty, 47 Domneasca Str., 800008, Galati, Romania 3 University Politehnica of Bucharest, 313 Splaiul Independentei, 060042, Bucharest, Romania 4 University Lucian Blaga Sibiu, Faculty of Medicine, 2A Lucian Blaga Str., 550169, Sibiu, Romania 5 Titu Maiorescu University, Faculty of Pharmacy, Pharmacognosy Phytochem Phytoterapy Department, 16 Gheorghe Sincai Str., Bucharest, Romania This study evaluates and compares by dye penetration method and SEM photomicrographs the sealing obtained using two different classes of adhesive systems (etch-and-rinse and self-etch with selective etching) with SDR (Dentsply) bulk fill composite. 84 class V cavities were prepared on oral and vestibular face of 42 intact, freshly extracted wisdom teeth. The cavities were randomly divided in two groups and restored: Group 1 with prime&bond one select (Dentsply) and SDR (Dentsply) and Group 2 with prime&bond one Etch&Rinse (Dentsply) and SDR (Dentsply). Prime&bond one Select (Dentsply) is a single component adhesive and can be used in self etch mode, in selective enamel etch mode, or in etch-and-rinse mode. We chosen for this study the selective etch of the enamel mode. Prime&bond one Etch Rinse (Dentsply) is a universal etch-and-rinse one-bottle dental adhesive, designed to be used in two steps. The bulk fill composites are commonly used in modern dentistry due to their properties of low polymerization shrinkage and curing in layer of 4 mm depth, offering the practitioner a fast clinical procedure with good results. The results showed a good sealing at enamel and dentin margins with no statistically significant difference between adhesives, even though the mean of enamel infiltration was smaller for Group1. Furthermore the results show that there were differences between the two groups, for the infiltrations at the enamel, the values of microleakage being arithmetically higher for Group 1, but with no statistically difference between the two groups.SEM images showed for both groups a good adhesion surface with the tooth, but the hybrid layer of the total-etch adhesives is different from the hybrid layer formed by self etch adhesives, in terms of thickness, uniformity. In conclusion both adhesive systems have equivalent sealing qualities and can be successfully used with SDR. Keywords : adhesive systems, polymerization shrinkage, infiltrations *email: erar_dr.kamel@yahoo.com; loredana_cc@yahoo.fr All authors have participated equally in developing this study. daniela_raiciu@yahoo.com Resin based composites are universally used for all types of cavities in anterior and posterior teeth. They are considered to be the main option of countless dentists in direct restoration of carious lesions, because of their advantages as high strength, low thermal conductivity, modulus elasticity, hardness [1, 2]. However, the drawbacks of this material are well known among the practitioners. Two of the principal limitations of composites are the cure depth and polymerization shrinkage . Consequently it is suggested the use of a layering technique, in which the layers should not exceed 2.5 mm, in order to obtain an appropriate polymerization [3]. Nowadays dentists use materials that decrease the chair time, reduce microleakage and increase patients compliance. In literature microleakage is defined as the clinically undetectable passage of bacteria, fluids, molecules or ions between a cavity wall and the restorative material [4-7]. Therefore the bulk-fill composites have been developed, in order to minimize the time of work required by the incremental techniques and decrease micro- leakage [2, 4]. Bulk-fill composites can be applied in layers of 4-5 mm, because of their high reactivity to light curing and reduced polymerization stress. They have larger filler size, less filler content, grater translucency than conventional composites and excellent bond strength regardless of the method of filling and cavity form. Depending on their consistence, there are available two types of bulk-fill composites: flowable and regular. With the regular type one can restore the whole cavity, while the flowable type must be completed by a 1.5 mm layer of conventional composite, in order to increase the aesthetics and the resistance to masticator forces [1, 4, 8]. The shrinkage that accompanies the polymerization of the composite resins causes stress at the interface tooth- restoration that can lead to microleakage, secondary or recurrent caries and pulpal irritation [3, 10, 11].The resulted stress should be absorbed by the adhesive systems. The development of adhesive materials provided an important step in restorative dentistry, that led to what is called today, minimal invasive dentistry [9, 12, 13]. Over the years dental adhesives have been classified in numerous ways, based on generations, on the components, on the number of steps or on the clinical strategy. Contemporary classifications depend on the clinical approach and divide adhesive systems into two groups: etch-and-rinse adhesives and self-etch adhesives [13-15]. Etch-and-rinse adhesive systems have two versions used in practice: in three steps and in two steps. In the three steps version, the primary components ( etchant, primer and bonding) are packaged, each one, in different bottles and the application is made in sequences, whereas the two steps version is a simplified version, that combines two of the components (primer and bonding) in one bottle,