Potato Virus Y NTN : A Coat and P1 Protein Sequences Analysis of a Brazilian Isolate Haiko Enok Sawazaki & José Alberto Caram de Souza-Dias & James H. Lorenzen & Colin Jeffries & Diego Grando Módolo & Dagmar Stach Machado Received: 27 November 2008 / Accepted: 26 November 2009 / Published online: 5 February 2010 # EAPR 2010 Abstract Potato tubers (Solanum tuberosum L. cv. Monalisa) showing superficial necrotic rings typical of Potato virus Y NTN (PVY NTN ) infection were collected from commercial potato fields in the State of São Paulo (Brazil). An isolate named IAC- PVY NTN was characterized by sequencing 822 and 836 nucleotides from the coat protein and P1 protein coding genes, respectively. The IAC-PVY NTN was observed as a European/North American recombinant isolate closely related to three Eu- PVY NTN isolates that share an alternative recombination site in the coat protein cistron near position 144. Therefore, the IAC-PVY NTN isolate may represent a novel Potato Research (2009) 52:379–392 DOI 10.1007/s11540-009-9141-6 H. E. Sawazaki : D. G. Módolo Centro de Pesquisa e Desenvolvimento de Recursos Genéticos Vegetais, APTA-Instituto Agronômico (IAC), C. Postal 28, CEP 13001-970 Campinas, SP, Brazil H. E. Sawazaki e-mail: henok@iac.sp.gov.br D. G. Módolo e-mail: diegograndom@yahoo.com.br J. A. C. de Souza-Dias (*) Centro de Pesquisa e Desenvolvimento de Fitossanidade, APTA-Instituto Agronômico (IAC), C. Postal 28, CEP 13001-970 Campinas, SP, Brazil e-mail: jcaram@iac.sp.gov.br J. H. Lorenzen Department of Plant Soil and Entomological Sciences, University of Idaho, Moscow, ID 83844, USA e-mail: jiml@uidaho.edu C. Jeffries SASA, Roddinglaw Road, Edinburgh EH12 9FJ( Scotland, UK e-mail: colin.jeffries@sasa.gsi.gov.uk D. S. Machado Departamento de Microbiologia e Imunologia, Instituto de Biologia, UNICAMP, C. Postal 6109, CEP 13083-862 Campinas, SP, Brazil e-mail: dmachado@unicamp.br