Life Science Journal 2014;11(8) http://www.lifesciencesite.com 484 Tissue culture and some of the factors affecting them and the micropropagation of strawberry Ahsan A. Kadhimi 1, Arshad Naji Alhasnawi 2, Azhar Mohamad 3 Wan Mohtar Wan Yusoff 4 Che Radziah Binti Che Mohd. Zain 5 School of Biosciences & Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Malaysia 1,2,4&5 Ministry of Higher Education, Iraq 1 University Presidency, AL- Muthanna University, Iraq 2 Malaysian Nuclear Agency, Malaysia 3 Corresponding author email: kkkihsan@yahoo.com Abstract: The plant tissue culture refers to the cultivation of different part of the plant, I both single cell organ tissue and under sterile circumstances at the media and industrial expansion in several controlled circumstances. The method of the plant tissue culture plays a pivotal role in the second green revolution that gene modification and biotechnology can be used for improving the crop harvest and also superiority. Through using plant tissue culture methods plants can be attained from several explants over direct or indirect morphogenesis and by somatic embryogenesis. Direct morphogenesis refers to the manufacture of shoots from explants deprived of passing over callus (unorganised tissue) stage while indirect morphogenesis relates to the generation of shoots over the callus stage. The culture in which an organized form of growing may be incessantly got is mentioned to as an organ culture. The most significant types of organ cultures used for micropropagation are meristem cultures, shoot cultures, embryo cultures and isolated root cultures. Callus cultures, suspension or cell cultures, protoplast cultures or other cultures are clustered as unorganised tissue cultures. The method that was used was the plant tissue culture in the circulation of several plants, that is also the most significant way for strawberry production, to get the plants disease-free viral and harvest plants like the mother plant or the manufacture of new kinds with good potentials that help the turnout for the cultivation of this plant, that is considered by its high fruits nutritional value. Most plants are affected by the micropropagation strawberry essential basics of the media, like hormones, vitamins, amino acids and physical state and the power source to the media. This paper is a summary of the classic and new technology plant tissue culture and the effect of some features, like the media and development managers (hormone plant), vitamins, amino acids and the physical case of the media. It frequently affects most of these features in the circulation of plants using the tissue culture over the effect in the creation of callus or embryos or vegetative emergence and development of roots, as well as in the fraction development of branches and roots as well as the development highlighted in the influence of these influences on the micropropagation of plants. [Ahsan A. Kadhimi Arshad Naji Alhasnawi Azhar Mohamad Wan Mohtar Wan Yusoff Che Radziah Binti Che Mohd. Zain. Tissue culture and some of the factors affecting them and the micropropagation of strawberry. Life Sci J 2014;11(8):484-493]. (ISSN:1097-8135). http://www.lifesciencesite.com . 63 Key words: tissue culture, micropropagation, Growth regulators, Amino acids, in vitro. 1. Introduction Tissue culture considered as an in vitro aseptic culture of cells, tissues, organs or whole plant below exact nutritional and ecological circumstances (Thorpe, 2007). that can be used for manufacturing clones of plants. The resulting clones are true-to kind of a selection of genotype. The organized circumstances often offer the culture of the environment helpful for their development and development. These circumstances have complete quantity of nutrients, pH medium, suitable temperature and complete gaseous and liquid condition. Plant tissue culture method can function for large amount of plant production. In addition to their usage as an instrument for several researches, during the recent years; plant tissue culture methods, have been used as an important industrial circumstances for the plant propagation, disease removal, plant development and manufacturing the secondary metabolites. Small bits of tissue (termed explants) are usable for manufacturing hundreds and thousands of plants in a nonstop procedure. One explant may be multiplied into different thousand plants in fairly short while period under controlled circumstances, regardless of the season and climate on a year round base (Akin-Idowu at all, 2009). Endangered, threatened and rare types have positively been grown and preserved by micropropagation as high number of development and small loads on number of preliminary plants and space. Additionally, plant tissue culture recognized as the most effective method for improving the crops by producing somaclonal and gametoclonal variations. The micropropagation method has a huge potential for manufacturing the plants with higher quality, separated from efficient variations in