J. Physi l. (1987), 384, pp. 27-37 27 With 5 text-figures Printed in Great Britain VOLTAGE-CLAMP ANALYSIS OF SOMATIC y-AMINOBUTYRIC ACID RESPONSES IN ADULT RAT HIPPOCAMPAL CAl NEURONES IN VITRO BY T. J. ASHWOOD*, G. L. COLLINGRIDGEt, CAROLINE E. HERRONt AND H. V. WHEAL From the Department of Neurophysiology, University of Southampton, Bassett Crescent East, Southampton S09 3TU and the tDepartment of Pharmacology, University of Bristol, University Walk, Bristol BS8 lTD (Received 7 February 1986) SUMMARY 1. The response of CAI pyramidal neurones to somatic application of y- aminobutyric acid (GABA) was studied in adult hippocampal slices using single- electrode voltage-clamp techniques. 2. Small ionophoretic applications of GABA produced a pure outward current at the cell resting potential when recording with potassium-acetate-filled micro- electrodes. This response reversed at a membrane potential of -69+5 mV (mean+ 1 S.D.; n = 20). In recordings made with caesium-chloride-filled electrodes the GABA response reversed at -24 + 12 mV (n = 9). 3. The effect of different holding potentials on the size of the GABA response was examined in the range of -100 to -40 mV in twenty neurones using potassium- acetate-filled electrodes. In every case outward rectification of the response was observed. For twelve neurones the mean ratio (±1 S.D. of the mean) of the conduc- tance increase produced by GABA at -55 mV compared to -85 mV was 1-9 + 0 5. 4. Step changes in holding potential resulted in shifts in chloride equilibrium potential (EC,), as determined by time-dependent changes in the size of GABA- induced currents. The new value of EC1 was generally reached within a few seconds of altering the membrane potential. Shifts in EC, did not appear to affect the extent of rectification but would cause underestimates of conductance measurements unless these were 'instantaneous'. The mean ratio (± 1 S.D. of the mean) of the 'instan- taneous'conductance increase produced by GABA at 13 mV positive to that at 13 mV negative to EC1 was 1-8 + 0 3. 5. The outward rectification was greater than that predicted by the constant-field equation. Possible factors that might contribute towards the rectification and its physiological significance are discussed. INTRODUCTION Outward rectification of chloride currents activated by y-aminobutyric acid (GABA) has been observed in a variety of non-mammalian preparations (Dudel, 1977; Onodera & Takeuchi, 1979; Dudel, Finger & Stettmeier, 1980; Cull-Candy, * Present address: The Astra Clinical Research Unit, 10, York Place, Edinburgh EHI 3EP. ) by guest on July 10, 2011 jp.physoc.org Downloaded from J Physiol (