Medikes (Media Informasi Kesehatan),Volume 7, Nomor 2,November 2020 335 Uji Daya Hambat Antibakteri Kapang Endofit dari Tanaman Alang-Alang (Imperata cylindrica (L.) Beauv.) terhadap Pertumbuhan Bakteri Staphylococcus aureus dan Escherichia coli Antibacterial Inhibition Test of Endophytic Fungi from Alang-Alang Plants (Imperata cylindrica (L.) Beauv.) on Bacterial Growth of Staphylococcus aureus and Escherichia coli Makhabbah Jamilatun 1 , Aminah Aminah 2 , Shufiyani Shufiyani 2 . 1 Poltekkes Kemenkes Surakarta, JL. Ksatrian, Danguran, Klaten 2 Poltekkes Kemenkes Banten, Jl. Dr. Sitanala, Neglasari Kota Tangerang Korespondensi : makhabbah.j@gmail.com ABSTRACT Attention to endophytic microbes has increased because it has several functions, including producing various functional compounds of secondary metabolites. Based on previous research, two endophytic fungi were isolated from the Imperata cylindrica (L.) Beauv., namely Fusarium sp. and Mucor sp. This research was conducted with the aim to determine the antibacterial activity of endophytic fungi from Imperata cylindrica (L.) Beauv.) on the growth of Staphylococcus aureus and Escherichia coli. The research began with rejuvenating and fermentation of endophytic fungi, then testing the antibacterial activity of fermentation from endophytic fungi carried out by the Kirby Bauer method. Based on the results of the study that endophytic fungi can inhibit the growth of test bacteria. Fusarium sp endophytic fungi were able to inhibit the growth of S. aureus and E.coli test bacteria with inhibition diameters of 16.2 mm and 16.8 mm, respectively. While the endophytic fungi of Mucor sp were able to inhibit the growth of S. aureus and E. coli test bacteria with inhibition diameters of 16.2 mm and 15.2 mm in Mucor sp isolates from stems, and 21.3 mm and 16.3 mm in Mucor sp isolates originating from the root. Based on the diameter of inhibitory zones formed, the antibacterial activity of endophytic fungi of Imperata cylindrica (L.) Beauv. is included in the strong category, so that secondary metabolites of endophytic fungi from Imperata cylindrica (L.) Beauv. have the potential to be developed and can be used as a reference source in the search for new types of antibiotics. Keywords: Endophytic Fungi, Fusarium sp., Mucor sp ABSTRAK Perhatian terhadap mikroba endofit telah meningkat karena mempunyai beberapa fungsi, antara lain dapat menghasilkan berbagai senyawa fungsional metabolit sekunder. Berdasarkan penelitian sebelumnya, dua kapang endofit berhasil diisolasi dari tanaman alang-alang (Imperata cylindrica (L.) Beauv.). yaitu Fusarium sp. dan Mucor sp. Penelitian ini dilakukan dengan tujuan untuk mengetahui aktivitas antibakteri kapang endofit dari tanaman alang- alang (Imperata cylindrica (L.) Beauv.) terhadap pertumbuhan bakteri Staphylococcus aureus dan Escherichia coli. Penelitian diawali dengan meremajakan dan fermentasi kapang endofit,