World Applied Sciences Journal 8 (9): 1056-1059, 2010 ISSN 1818-4952 © IDOSI Publications, 2010 Corresponding Author: Dr. Kamaruzaman Jusoff, Faculty of Forestry, Universiti Putra Malaysia, 43400 Serdang, Selangor. Malaysia. Tel: +609-460 2780 1056 Laevifonol: A Unique Dimer Oligostilbene from the Stem Bark of Vatica odorata Wan Zuraida Wan Mohd Zain and Kamaruzaman Jusoff 1 2 Chemistry Department, Faculty of Applied Sciences, 1 Universiti Teknologi MARA Pahang, 26400 Jengka, Pahang, Malaysia Faculty of Forestry, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia 2 Abstract: The isolation of laevifonol (dimerstilbene) in Vatica odorata is the second time for its present in Vatica sp. This compound is a unique oligostilbene formed from a condensation between g-viniferin and ascorbic acid and was firstly isolated from Shorea laevifonia and recently from Vatica umbonata. The structure of laevifonol was established on the basis of their spectral data, including UV, IR and NMR spectra and also in comparison with the previously reported data. Cytotoxic properties was evaluated against murine leukimia P-388 cells and Artemia salina which resulting not strongly inhibited with IC values of 60.5 μM and 50 > 796.2 μM, respectively. Antibacterial activity also was screened against two gram positive bacteria (Bacillus subtilis and Staphylococcus aeureus) and one gram negative bacteria (Escherichia coli). The antibacterial testing was carried out by using the disc diffusion method. Blanc disc of 6mm diameter was loaded with 1000 μg/ml of the compound applied to the inoculate plate. The compound showed moderate activity against all the bacteria with inhibition zones of 0.5 mm against E.coli and B.subtilis and 0.1 mm against S. aerues compared to positive control (Erythromycin 60 μg) with 0.13 mm, 0.03 mm and 0.1 mm each. The present investigation is apart of our ongoing studies on the oligostilbene of Malaysian Dipterocarpacaeae in which no phytochemical data was recorded on V. odorata. Key words: Oligostilbene % Dipterocarpaceae % Vatica odorata % Laevifonol % Biological activity INTRODUCTION MATERIALS AND METHODS Stilbene (3,4’,5-trihydroxystilbene) is a phenolic General Experimental Procedures: UV spectra were compound are occuring in the particular families such as measured with a Varian Conc. 100 instrument. IR spectra Dipterocarpaceae, Vitaceae, Cyperaceae, Leguminoseae were determined with a Perkin Elmer FTIR Spectrum One and Gnetaceae [1]. Vatica is a relatively large genus spectrometer using KBr pellets. H and C NMR spectra belonging to the family Dipterocarpaceae and is were recorded with a JEOL ECP400 operating at 400 ( H) distributed mainly in Southest Asia [2]. Genus Vatica, and 100 ( C) MHz using residual and deuterated solvent Shorea, Hopea and Vateria have proven to be a rich peaks as reference standards. Vacuum liquid (VLC) and source of oligostilbene compounds derived from a column chromatography were carried out using Merck stilbene [3]. As a phytoestrogen, oligostilbene has silica gel 60 GF and silica gel G60 35-70 mesh. For TLC recently been drawn to attentions because of its various analysis, precoated silica gel plates (Merck Kieselgel biological properties that may account for its possible 60 GF , 0.25 mm) were used. cardioprotective action, including of smooth muscle cell proliferation and platelet aggregration [4]. Moreover, this Plant Materials: Samples of the stem barks of V.odorata compound shows anti-inflammatory [5] and anticancer were collected from Pulau Mata Kail, Belum Forest activities [6]. Because of its pharmacological functions, Reserve, Perak, Malaysia. The plant was identified by oligostilbene is now gaining scientific attention as a botanist, University Putra Malaysia and a voucher longevity promoter. specimen was deposited in the herbarium (SK 616/03). 1 13 1 13 254 254