_____________________________________________________________________________________________________ *Corresponding author: E-mail: zaccheusshehu@gmail.com; Journal of Pharmaceutical Research International 32(34): 31-39, 2020; Article no.JPRI.63445 ISSN: 2456-9119 (Past name: British Journal of Pharmaceutical Research, Past ISSN: 2231-2919, NLM ID: 101631759) Bio-Fabrication of ZnO-CuO Nanoporous Composite and Its Application as Nanolarvicidal Agent for Malaria Vectors Zaccheus Shehu 1* , Ezra Abba 2 , Danbature Wilson Lamayi 1 , Kennedy Poloma Yoriyo 2 , Zainab Adamu Abubakar 3 , Zungi Namau Kenneth 2 , Zirmba Usiju 2 and Amina Abubakar 3 1 Department of Chemistry, Faculty of Science, Gombe State University, Nigeria. 2 Department of Zoology, Faculty of Science, Gombe State University, Nigeria. 3 Department of Biology, Faculty of Science, Gombe State University, Nigeria. Authors’ contributions This work was carried out in collaboration among all authors. Authors ZS, EA, DWL and KPY designed the study, performed the statistical analysis, wrote the protocol and wrote the first draft of the manuscript. Authors ZAA, ZNK, ZU and AA managed the analyses of the study. All authors read and approved the final manuscript. Article Information DOI: 10.9734/JPRI/2020/v32i3430962 Editor(s): (1) Dr. Jongwha Chang, University of Texas, USA. Reviewers: (1) Ahmed Mohammed Abu-Dief Mohammed, Egypt. (2) Arpita Chatterjee, Barasat College, West Bengal State University, India. Complete Peer review History: http://www.sdiarticle4.com/review-history/63445 Received 20 September 2020 Accepted 24 November 2020 Published 10 December 2020 ABSTRACT Bio-fabrication of ZnO-CuO nanoporous composite was successfully synthesized using Gum Arabic. The morphology, elemental composition, surface plasmon resonance and functional groups bonds of the ZnO-CuO nanoporous composite were confirmed by SEM, EDX, UV-Visible and FTIR techniques respectively. Nanolarvicidal activity of ZnO-CuO nanoporous composite was tested against 1 st , 2 nd , 3 rd , and 4 th instars of malaria vectors for 24 hours using different concentrations. The lethal concentrations (LC 50 ) of ZnO-CuO nanoporous composite against 1 st , 2 nd , 3 rd , and 4 th instars were found to be 8.841, 8.734, 8.963 and 10.557 mg/L respectively. Whereas, the LC 90 of ZnO-CuO nanoporous composite against 1 st , 2 nd , 3 rd , and 4 th instars were found to be 19.062, 28.063, 40.888 and 79.567 mg/L respectively. For all instars, the correlation coefficients were found Original Research Article