Salmonellosis and Shigellosis Associated with Catle Dung Contaminant from Indigenous Abatoirs, Osun State, Nigeria Joseph Omololu-Aso * , Oluwaseun Oluwatoyin Omololu-Aso, Micheal T Atiene, Adekunle Adejuwon, Alexandrer Tuesday Owolabi and Arwa Shesha Department of Microbiology, Obafemi Awolowo University Ile-Ife, Osun Nigeria * Corresponding author: Joseph Omololu-Aso, Obafemi Awolowo University Ile-Ife, Osun Nigeria, Tel: +234-8033770933; E-mail: omololu- aso@oauife.edu.ng Received Date: February 04, 2017; Accepted Date: February 10, 2017; Published Date: February 15, 2017 Copyright: © 2017 Omololu-Aso, et al. This is an open-access artcle distributed under the terms of the Creatve Commons Atributon License, which permits unrestricted use, distributon, and reproducton in any medium, provided the original author and source are credited. Citaton: Omololu-Aso J, Omololu-Aso OO, Atene MT, et al. Salmonellosis and Shigellosis Associated with Catle Dung Contaminant from Indigenous Abatoirs, Osun State, Nigeria. Br J Res 2017, 4: 1. Abstract Faecal shedding of catle as well as poultry and poultry products have been implicated as a major source of Salmonella infectons in humans. We sourced to isolate and identfy Salmonella and Shigella species from catle dung in diferent abatoir of Ile-Ife, Nigeria and to detect the route of cross-contaminaton of Salmonella and Shigella from animals to humans with antbiotc susceptbility of the isolates recovered. All 40 samples taken were collected from the dung of freshly killed cows which were placed within sterile plastc containers and transported to the Microbiology Laboratory. Each Sample was placed aseptcally in sterile nutrient broth and incubated. Microbiological analysis was conducted with samples serially diluted and analyzed on Salmonella-Shigella agar by the direct platng method. Biochemical tests were employed to confrm the isolates including Gram staining, catalase, oxidase, urease, glucose fermentaton and motlity tests. The results showed that out of the ten (10) samples collected in Odo-Eran area, Ile-Ife town, 4 (40%) samples showed Escherichia coli growth, 4 (40%) Salmonella were isolated and 2 (20%) Shigella-like organisms were isolated. At God's Love Tabernacle (GLT) area in Ile Ife town, From the 20 samples taken, 7 (35%) showed Salmonella isolates, 7 (35%) confrmed Shigella-like characteristcs isolates. Six (30%) isolates lef were observed to show Escherichia coli colonies. The Salmonella/Shigella isolates obtained from study areas were 100% resistant to chloramphenicol, 75% to septrin and 10% to pefoxacin and tarivid respectvely while the isolates were 80% susceptble to gentamicin and 65% to ciprofoxacin and streptomycin used. Gentamicin streptomycin and ciprofoxacin antbiotc therapeutc agents will be efectve in the treatment of catle infectons caused by Salmonella and Shigella species in the study area. Epidemiological burden of Salmonellosis is expected to be much greater in the developing parts of the world. Keywords: Salmonella-Shigella; Fecal samples; Antbiotcs; Abatoir; Infecton; Contaminant Introducton Salmonella are the leading cause of bacteremia, 28.5% with S. typhi accountng for 20.9% and non-typhi salmonella 7.6% [1]. Infectons due to salmonella serotypes contnue to be a major health problem [2]. Worldwide incidence of shigellosis is estmated to be 164.7 million cases per year of which 163.2 million were in developing countries, where 1.1 million deaths occurred. About 60% of all episodes and 61% of all deaths atributable to shigellosis involved catle and children below fve years. The incidence in developing countries may be 20 tmes greater than that in developed countries [3]. An estmated 30% incidence of these infectons are caused by S. dysenteriae with case fatality rates reaching 30% [3]. Salmonellosis is one of the most common and widely distributed food-borne diseases. It consttutes a major public health burden and represents a signifcant cost in many countries [4]. Most Salmonella infectons in catle are subclinical, and shedding of the organism can occur for extended periods. Salmonella can also persist in the farm environment [5]. Shigella species are highly infectve, partcularly S. dysenteriae considered the most virulent, and can produce a potent cytotoxin known as “Shiga toxin”. Shigella dysenteriae type 1 causes severe and sometmes fatal disease. Shigella species have been recently identfed to be the most frequently identfed agent of laboratory-acquired infectons because of their high virulence and low infectous dose [6]. In Nigeria, morbidity associated with illnesses due to Salmonella contnues to be on the increase and in some cases resultng in deaths [7]. It is common in developing countries where afected populatons are immunologically compromised due to poor nutriton and background infectons leading to high morbidity atributed to shigellosis [8-10]. Salmonellosis, a major cause of bacteria enteric illness in both humans and animals cause diseases in humans and is referred to as a zoonotc infecton/disease. It is a disease caused by the bacteria Samonella [4]. Research Article iMedPub Journals http://www.imedpub.com/ DOI: 10.21767/2394-3718.100002 British Journal of Research ISSN 2394-3718 Vol.4 No.1:2 2017 © Under License of Creative Commons Attribution 3.0 License | This article is available from: http://www.imedpub.com/british-journal-of-research/ 1