Int.J.Curr.Microbiol.App.Sci (2017) 6(3): 530-540 530 Original Research Article https://doi.org/10.20546/ijcmas.2017.603.062 Green Synthesis and Characterization of Silver Nanoparticles from Gymnema sylvestre Leaf Extract: Study of Antimicrobial Activities N. Supraja 1 , B. Avinash 2 and T.N.V.K.V Prasad 1 * 1 Acharya N. G. Ranga Agricultural University, Regional Agricultural Research Station, Tirupati-517502, A.P. India 2 College of veterinary science Tirupati, Sri Venkateswara Veterinary University, Tirupati, India *Corresponding author ABSTRACT Introduction Uncontrolled growth and spread of abnormal cells lead to cancer and finally results in death. Ethno-pharmacological process on the synthesis of nanoparticles is an amazing technology beneath construction symbiosis between nanoscience and medical sciences. In this regard, the idea of functionalizing gold nanoparticles for antidiabetic nanomaterial, by synthesizing pharmacologically key plant materials often been considered. Advances beneath nanotechnology have identified possible candidates for biological and biomedical programs on pharmaceutics, for novel diagnostics and medical agents. The nanoparticle drug delivery system has the advantages of accumulating large amounts of therapeutic drugs in the tumor tissues through the passive and active targeting approach (Mateos et al., 2013). Colon adenocarcinoma is the most common type of gastrointestinal cancer, and the National Institute of cancer has reported about 140,000 cases each year in the United States. This type of cancer begins in the inner layers of glandular structures of the colon cells and spreads into the wall of the colon and potentially into the lymphatic system and other organs (Ebrahimnejad et al., 2010). Gymnema sylvestre (GS), a plant used in the Indian Ayurvedic medicine for treating diabetes mellitus, has been known from antiquity also to have an anti saccharin taste effect. Many researchers have reported that International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 6 Number 3 (2017) pp. 530-540 Journal homepage: http://www.ijcmas.com A versatile technique was implemented for the synthesis of silver nanoparticle using aqueous extract of leaves of Gymnema sylvestre. The reduction of silver nitrate to silver nanoparticle was confirmed by UV-Vis spectrophotometer showing a typical resonance (SPR) at about 390nm which is specific for ag NPs. In FT-IR analysis the strong band peak at 3355cm -1 in aqueous extract is vibration bands, which may be due to overlapping of amine N- H stretching bands. HR-SEM images reveal that the particles are spherical and relatively uniform shape of the silver nanoparticles was confirmed in the range of 30- 50nm in aqueous extract, whereas DLS shown good zeta potential and less particle size finally silver nanoparticle using aqueous extract of leaves of Gymnema sylvestre shown very high antimicrobial activity. Keywords Gymnema sylvestre, Silver nanoparticles, Antimicrobial activity (Fungi and Bacteria). Accepted: 10 February 2017 Available Online: 10 March 2017 Article Info