_____________________________________________________________________________________________________ *Corresponding author: Email: hendahmed2@hotmail.com; European Journal of Nutrition & Food Safety 8(4): 148-154, 2018; Article no.EJNFS.2018.024 ISSN: 2347-5641 Detection of Oxytetracycline Residues in Table Eggs in Khartoum State, Sudan E. A. Hind 1* , K. M. Osman 2 , Ishraga, G. Ibrahim 3 and Y. A. Sabiel 1 1 Department of Bacteriology, Central Veterinary Research Laboratory, Khartoum, Sudan. 2 Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Khartoum, Sudan. 3 Department of Biochemistry, Pharmacology and Toxicology, Central Veterinary Research Laboratory, Khartoum, Sudan. Authors’ contributions This work was carried out in collaboration between all authors. Author EAH designs the study, wrote the protocol and the first draft of the manuscript and conduct the laboratory analysis of the samples. Author KMO supervised design the study and managed the literature searches. Author IGI performed the statistical analysis and supervised the quantitative analysis. Author YAS supervised the qualitative analysis of the sample and revised the manuscript. All authors read and approved the final manuscript. Article Information DOI: 10.9734/EJNFS/2018/39827 Received 31 st January 2018 Accepted 21 st May 2018 Published 28 th May 2018 ABSTRACT Aims: This study was conducted to detect the presence of oxytetracycline residues in table eggs in Khartoum State and to compare its level with the international acceptable maximum residue limits (MRLs). Study Design: One hundred and eighty table egg samples were randomly collected from 18 sale points in the three localities of Khartoum State, Sudan, (60 eggs from Omdurman, 60 eggs from Khartoum and 60 eggs from Khartoum North). Place and Duration of Study: Samples collected from Khartoum State during August and September, 2015. Methodology: Microbiological inhibition assay was used to screen the presence of antibiotic residues using Bacillus subtilis seeded in nutrient agar. Ninety positive egg samples from the microbiological inhibition assay were analyzed to detect the presence and quantity of oxytetracycline residues using HPLC. Results: Microbiological inhibition assay showed that 50% of the tested samples were positive for antibiotic residues in Omdurman, Khartoum and Khartoum North with 34(18.9%), 28(15.6%), and 28(15.6%) of the antibiotics respectively. HPLC results showed that 63(70%) were positive for oxytetracycline residues 19(10.5%) from Omdurman, 21(11.6%) from Khartoum and 23(12.7%) from Khartoum North. Original Research Article