Thursday, August 29, 2013 FREE COMMUNICATIONS SESSIONS 17–32 Free Communication Session 17 | B332 | 29.08.2013 | 09:00–11:00 Theme: Preventive Dentistry: Orthodontics FC100 Antimicrobial Efficacy of Salvadora persica Extracts on Orthodontic Brackets Hassan Suliman Halawany 1 , Nimmi Biju Abraham 2 , Yunus Mohammed Siddiqui 1 , Hanan Abdulgafur Balto 3 , Vimal Jacob 1 1 Department of Periodontics and Community Dentistry, College of Dentistry, King Saud University, Riyadh, Saudi Arabia, 2 Dental Caries Research Chair, College of Dentistry, King Saud University, Riyadh, Saudi Arabia, 3 Division of Endodontics, Department of Restorative Dental Sciences, College of Dentistry, King Saud University, Riyadh, Saudi Arabia Aim: The aim of the study was to investigate in vitro, the antimicro- bial potential of hexane and ethanol extracts of Salvadora persica on a monospecies-biofilm model established on orthodontic brackets. Materials and methods: Streptococcus mutans biofilm was formed on 48 orthodontic brackets following 3 days incubation at 37°C in anaerobic condition. The bacterial cell viability of this biofilm was measured after their exposure to saline, hexane extract of Salvadora persica, ethanol extract of Salvadora persica and 0.2% chlorhexidine using 3-(4, 5-dimethylthiazol-2-yl)-5-(3-carboxy- methoxyphenyl)-2-(4-sulphophenyl)-2H-tetrazolium (MTS) assay. Half of the brackets were quantified by enumeration of the colony forming units (CFU). The absorbance values obtained from the MTS reduction assay were analyzed statistically using one-way analysis of variance comparing the test groups with the controls. Values of p < 0.05 were considered statistically significant. Results: The absorbance values obtained from the MTS assay showed that the cell viability of Streptococcus mutans biofilm when exposed to 5 mg/ml of hexane extract <5 mg/ml ethanol extract <saline. The cell viability did not differ significantly when exposed to hexane extract and chlorhexidine separately (p > 0.05). The CFU counts of Streptococcus mutans obtained from chlorhexidine exposure were lower when compared to those obtained from hexane and ethanol extract exposure. Conclusions: Hexane extract of Salvadora persica was found to have a high antimicrobial efficacy while the ethanol extract showed a moderate efficacy on Streptococcus mutans biofilm established on orthodontic brackets suggestive of its potential use as an oral antimicrobial agent for orthodontic patients. FC101 Assessment of Fluctuating Asymmetry in Various Malocclusions Saima Nizar Hirji Aga Khan University Hospital, Nairobi, Kenya Aim: Assessment of fluctuating asymmetry has clinical importance in the diagnosis and treatment planning of orthodontic patients. Analyzing presence of fluctuating asymmetry in various malocclu- sion, helps in recognition of asymmetry, for its input in treatment planning. Objective: To assess fluctuating asymmetry in Angle Class I and Class II malocclusions. To assess pattern of fluctuating asymme- try in males and females in Angle Class I and Class II malocclusions. Materials and methods: Its a cross sectional study conducted in dental clinic, AKUH during the period July 2010–July 2011 on 100 subjects using non probability purposive sampling technique. Digital vernier caliper is used as tool to measure mesiodistal and buccolingual dimensions of central incisors, canines and first molars in maxillary and mandibular arch. Results: Fluctuating asymmetry was observed in both Class I and Class II malocclusions. Pattern of fluctuating asymmetry was dif- ferent in males and females in Class II malocclusion. Conclusion: Pattern of asymmetry is same in both Class I and Class II malocclusion, although gender dimorphism is seen in Class II malocclusion. FC102 Camoflauge- A Viable Option for Treatment of Skeletal Class III Malocclusion Vaishali Devidas Vadgaonkar 1 , Girish Karandikar 2 , Anita Karandikar 3 , Parag Gangurde 1 , Rahul Hegde 4 , Varsha Rathod 5 , Rajesh Koppikar 6 , Arun Nayak 1 , Manthan Patel 1 , Aniket Gandhi 1 , Nikhil Mahajan 1 , Pallavi Shinde 1 1 Department of Orthodontics and Dentofacial Orthpaedics, Bharati Vidyapeeth Deemed University Dental College and Hospital, Navi Mumbai, India, 2 Department of Orthodontics and Dentofacial Orthpaedics, M.G.M. Dental College and Hospital, Navi Mumbai, India, 3 Department of Orthodontics and Dentofacial Orthpaedics, Yerla Medical Trust Dental College and Hospital, Navi Mumbai, India, 4 Department of Pedodontics and Preventive Dentistry, Bharati Vidyapeeth Deemed University Dental College and Hospital, Navi Mumbai, India, 5 Department of Periodontology, Bharati Vidyapeeth Deemed University Dental College and Hospital, Navi Mumbai, India, 6 Department of Periodontology, Bharati Vidyapeeth Deemed University Dental College and Hospital, Sangli, India Treatment of Skeletal Class-III can be done by three modalities: Conservative, Camouflage and Surgical. In this paper we are describing Camouflage as a treatment modality for correction of Skeletal Class-III. There are several advantages of camouflage treatment such as: it is Non-Surgical, Apprehension Free, Patient Friendly, Slow but Steady and economical. Decision for camouflage treatment depends mainly upon two main factors: Patient factors and Ortho- dontist factors. International Dental Journal 2013; 63 (Suppl. 1): 99--197 © 2013 The authors. International Dental Journal © 2013 FDI World Dental Federation 99