Simplified cell culture method for the diagnosis of atypical Primary Ciliary Dyskinesia Massimo Pifferi 1 , Francesca Montemurro 2 , Angela M. Cangiotti 3 , Vincenzo Ragazzo 1 , Maria Di Cicco 1 , Bruna Vinci 2 , Giovanni Vozzi 2 , Pierantonio Macchia 1 , and Attilio L. Boner 4 1 Department of Pediatrics, University of Pisa, Italy 2 Interdepartmental Research Center "E. Piaggio", Faculty of Engineering, University of Pisa, Italy 3 Institute of Normal Human Morphology, Electron Microscopy Unit, Umberto I° Hospital, University of Ancona, Italy 4 Department of Pediatrics, University of Verona, Italy Correspondence and requests for reprints should be addressed to: Massimo Pifferi MD, PhD Department of Pediatrics University of Pisa Via Roma 67 56122 Pisa - Italy Fax: ++39 50 992641 Phone: ++39 50 992728 e-mail: m.pifferi@med.unipi.it Running Head: Simplified method for PCD diagnosis Body of the Manuscript Word Count: 2771 Key Words: ciliary dyskinesia, ciliary motion analysis, ciliogenesis, transmission electron microscopy. Thorax Online First, published on September 21, 2009 as 10.1136/thx.2008.110940 Copyright Article author (or their employer) 2009. Produced by BMJ Publishing Group Ltd (& BTS) under licence. on May 31, 2020 by guest. Protected by copyright. http://thorax.bmj.com/ Thorax: first published as 10.1136/thx.2008.110940 on 21 September 2009. Downloaded from