Supporting Information: Metal Affinity-Enabled Capture and Release Antibody Reagents Generate a Multiplex Biomarker Enrichment System that Improves Detection Limits of Rapid Diagnostic Tests Authors: Westley S. Bauer 1 , Christopher P. Gulka 1 , Lidalee Silva-Baucage 1 , Nicholas M. Adams 2 , Frederick R. Haselton 2 , and David W. Wright 1 * 1 Department of Chemistry, Vanderbilt University, Nashville, TN 37235 2 Department of Biomedical Engineering, Vanderbilt University, Nashville, TN 37235 *To whom correspondence should be addressed: david.wright@vanderbilt.edu, +1-615-322-2636 The contents of this document include supporting materials regarding the synthesis, purification, and characterization of the His 6 -peptides used in this study. Also provided are studies that determined the optimal antibody-peptide conjugation conditions, the method used to calculate theoretical antibody bead coverage, the compatibility of CaR mAb reagents with the selected RDT, and a representative RDT image with and without the use of the biomarker enhancement strategy.