International Journal of Ayurvedic Medicine, Vol 11 (3), 456-465 Published online in http://ijam.co.in ISSN No: 0976-5921 Research Article Key Words: Scorpion sting, Paravatadi Agada, Indian red scorpion venom, Anti-scorpion venom. Introduction Scorpion sting is a frequent event in tropical and subtropical countries (1). It is estimated that in India the annual number of scorpion stings cases exceeds 1.23 million, of which over 32,250 may be fatal. India is the most affected, with a reported incidence of 0.6% (2) These are found in large quantity in western Maharashtra, Parts of Karnataka, Andhra Pradesh, Saurashtra, Pondicherry and Tamil Nadu (3). In India severe scorpion sting due to Mesobuthus tamales species of scorpion is mainly found in Kokan region (4). Higher incidence of scorpion sting occurred during hot months attributed to increase in agricultural activity(5,6). Scorpion venom causes massive release of neurotransmitter which results in clinical features of envenomation (7). Clinical effects of stings are related to age, size of scorpion, season of the sting and time lapsed between sting and hospitalization (8). It is classified into local manifestation, systemic involvement, Cardiogenic failure (hypotension, ventricular arrhythmia, bradycardia, cardiovascular collapse) and Respiratory failure(cyanosis, dyspnoea, p u l m o n a r y o e d e m a ) ( 9 ) . I n A y u r v e d a Vrishchika(scorpion) are considered as one of the kita which is explained under the context of Kitvishpratishedha (10). Various Agada are mentioned in classic for different poisoning cases along with it can be prescribed in the condition other than poisonous incidence. (11). Paravatadi Agada (PA) is one of the remedy mentioned in Astang Hridayam for the treatment of scorpion bite poisoning and it is narrated that this formulation is best among all other remedies to treat scorpion bite (12). However, efficacy of PA has not been scientifically proved yet. Therefore, present work was undertaken with aim to evaluate the efficacy of PA in the management of Indian Red Scorpion bite on Albino mice. Materials and Methods Collection of Raw material All the ingredients were procured from local market Nagpur. Paravat Shakrut was collected from Poultry farmer, Wardha. Fruit of Bijapurak was collected from Kolhapur. All the drugs were authenticated by subject expert. Experimental Study on Anti Scorpion Venom potential of Paravatadi Agada of Ayurveda in Indian Red Scorpion Venom (Mesobuthus tamulus) Sunil Deshmukh 1 , Sonali Chalakh 2* , Dhirajsing Rajput 3 1. PG Scholar, 2. Professor, Department of Agad Tantra, 3. Associate Professor, Department of Rasashastra, Mahatma Gandhi Ayurveda College, Hospital and Research Centre, Datta Meghe Institute of Medical Sciences, Salod (H), Wardha, Maharashtra. Abstract Background: Scorpion sting is a frequent event in tropical and subtropical countries. The objective of this study is to evaluate Efficacy of Paravatadi Agada on Indian Red Scorpion Venom (Mesobuthus Tamulus). Materials and Methods: PA was prepared as per textual reference. Water soluble extract of PA was obtained using Soxhlet apparatus. Swiss albino mice of 20-30gm were used. Lypholised venom sample of Mesobuthus tamales and Lyophilized monovalent enzyme refined immunoglobulin anti scorpion venom serum (ASV) was used. Using lethal dose of scorpion venom (25.12 µg/g), venom neutralising property of PA extract (300mg/kg), ASV(1mg) intra- peritoneally and PA(31mg/mice) orally. The parameter used were Mean survival time, protection fold and percentage survival of animals over the period of 24 hrs. Histopathological examinations of all mice were done. Result: Maximum protection fold is seen in ASV treated group which is 10.03 with 83.33 % survival but water soluble extract of PA also showed some protective effect against scorpion venom 7.68 with 50 % survival rate. Histopathological examination showed that PA extract, ASV and PA treated group showed less effect of scorpion venom on Heart, Liver and Kidney compared to control group in which sever histopathological manifestations are detected. Conclusion : The protection fold and survival percentage of extract of PA was better than Powder form of PA but less than ASV but enough significant in view of availability, safety, ease in method of preparation and cost effectiveness compared to ASV. 456 * Corresponding Author: Sonali Chalakh Professor, Department of Agad Tantra, Mahatma Gandhi Ayurveda College, Hospital and Research Centre, Datta Meghe Institute of Medical Sciences, Salod (H), Wardha, Maharashtra, India Email Id: spchalakh@gmail.com