____________________________________________________________________________________________ *Corresponding author: Email: accessrohit25@gmail.com; British Microbiology Research Journal 3(4): 743-752, 2013 SCIENCEDOMAIN international www.sciencedomain.org Characterization of Lactic Acid Bacteria from Raw Milk Samples of Cow, Goat, Sheep, Camel and Buffalo with Special Elucidation to Lactic Acid Production Rohit Sharma 1,2* , Bhagwan S. Sanodiya 1 , Gulab S. Thakur 1 , Pallavi Jaiswal 1 , Sangeeta Pal 1 , Anjana Sharma 2 and Prakash S. Bisen 1,2 1 Microbial Biotechnology Laboratory, Tropilite Foods Pvt Ltd, Davars Campus, Tansen Road, Gwalior- 474002 (M.P), India. 2 Department of Post Graduate Studies and Research in Biological Sciences, Rani Durgavati Vishwavidyalaya, Jabalpur – 482004 (M.P), India. Author’s contributions This work was carried out in collaboration between all authors. Author RS and BSS were involved in experimental work, authors GST, PJ and SP were associated with analyses of the study, writing first draft of the manuscript, and authors AS and PSB were associated with study design, execution and manuscript writing. All authors read and approved the final manuscript. Received 13 th May 2013 Accepted 27 th August 2013 Published 6 th September 2013 ABSTRACT Aims: Selective enumeration, isolation and characterization of lactic acid bacteria from different raw milk sources with special elucidation to lactic acid production. Study Design: Serial dilution for strain isolation from raw milk samples to be done followed by complete morphological, biochemical and molecular characterization aiming to study the microbial behavior to ferment different sugar and producing lactic acid as end product. Place and Duration of Study: Microbiology Biotechnology Laboratory, Tropilite Foods Pvt. Ltd. Gwalior (M.P)-INDIA from Sep 2012 to April 2013. Methodology: Milk samples from cow, sheep, goat, camel and buffalo were collected from the surrounding area of Gwalior district of Madhya Pradesh, India, from local milk suppliers. 13 potent strains in terms of lactic acid production were selected for analysis. Gram Research Article