IJSRST207241 | Received : 10 March 2019 | Accepted : 22 March 2019 | March-April-2019 [ (6) 2 : 851-863 ] © 2019 IJSRST | Volume 6 | Issue 2 | Print ISSN: 2395-6011 | Online ISSN: 2395-602X doi : https://doi.org/10.32628/IJSRST218140 Themed Section: Science and Technology 851 Development and Validation of Absorption Correction UV- Spectrophotometry Method for Simultaneous Estimation of Ranitidine HCL and Dicyclomine Hydrochloride in Their Marketed Formulation Bhumi Kantariya*, Dr Greeva Bhatt, Dr. Mehul Mehta, Urmi Kanatria, Ravi Dalsaniya Lecturer Department of Pharmaceutical Chemistry, Indian Institute of Ayurvedic Pharmaceutical Sciences, Gujarat Ayurved University, Jamnagar, Gujarat, India ABSTRACT A Simple, selective, precise and rapid absorption correction Spectrophotometric method has been developed and validated as per ICH guideline for the simultaneous estimation of Ranitidine Hydrochloride and Dicyclomine Hydrochloride in tablet dosage form. Method is base on UV Spectrophotometric for determination of two drug, by using Methanol as a solvent and diluted the same with 0.1N NaOH, solution. In this UV method, the two wavelength were selected, 311.4 nm and at 217 nm for RANTD and DICY, respectively. this method was validated according to ICH guideline and Linearity range, was found to be 7.5-37.5 µg/ml and 1-5 µg/ml for RANTD and DICY, respectively. The method was successfully applied to assay drugs in tablet. Keywords :- UV Spectrophotometric absorption correction method; Ranitidine Hydrochloride (RANTD); Dicyclomine Hydrochloride (DICY); ICH guidelines; Validation. I. INTRODUCTION Ranitidine Hydrochloride N-(2-[(5- (dimethylaminomethyl) furan-2-yl) methylthio]ethyl)- N-methyl- 2- nitroethene- 1,1- diamine (Figure 1) is histamine H2 receptor antagonist. Dicyclomine Hydrochloride 2- (diethylamino) ethyl cyclohexylcyclohexane-1- carboxylate (Figure 2) is antiemetic, antimuscarinics and anticholinergic agent. Combination is frequently used in the treatment of acute ulcer.[32-37] A literature survey revealed there are many analytical methods reported for estimation of Ranitidine Hydrochloride & Dicyclomine Hydrochloride in individuals & in combination with other drugs in bulk and pharmaceutical dosage forms.[45-52] We have developed and validate new specific, very simple, accurate, precise spectrophotometry for estimation of marketed drug formulations. O S H N H N CHNO 2 H 3 C N H 3 C CH 3 Figure 1 : Ranitidine Hydrochloride O O N H 3 C H 3 C HCl