31 doi:10.5958/0974-0171.2019.00009.8 Indian J. Vet. Res. Vol. 28 No.2. 2019:31-35 Evaluation of combination of antibiotic with multienzyme preparation on bovine mastitis Mohsina Mushtaq * , Rajesh Agrawal, Nishi Pande and Rajiv Singh Division of Veterinary Medicine, SKUAST-J, R .S. Pura, Jammu, (J & K) *Corresponding author: mosu2011@gmail.com / mohsina042019@gmail.com [Received; 3 March 2020; Accepted 21 April 2020 ] Citation: Mohsina Mushtaq, Rajesh Agrawal, Nishi Pande and Rajiv Singh (2019) Evaluation of combination of antibiotic with multienzyme preparation on bovine mastitis. Indian J. Vet. Res. 2019, 28 (2):31-35. ABSTRACT The study evaluated the efficacy of multienzyme therapy as an adjunct or alternative to antibiotic therapy in mastitic cows. Twenty one cows affected with clinical mastitis were selected and divided into three treatment groups: Group 1 (n=7) animals were treated with enrofloxacin @ 10 mg/kg b.w, I/M for 5 days. Group 2 (n=7) cows received an intramammary infusion consisting of a mixture of three enzymes viz. chymotrypsin (8 mg), trypsin (8 mg) and papain (4 mg) dissolved in 5 ml NS, at 24 hr. interval for 7 days. Group 3 (n=7) cows received a combination of both parental antibiotic (Enrocin) and intramammary infusion of multienzymes for 7 days at 24 hours interval. Milk samples were collected prior to the treatment (day 1) and on days 4, 7 and 14 post treatment and blood samples on day 1, 7 and 14. There was significant (P<0.05) decrease in the mean serum total protein (TP) and globulin levels in all the treatment groups post treatment as compared to the pre- treatment levels while as the mean serum albumin levels and the A: G ratio showed significant (P<0.05) increase post treatment. There was a significant (P<0.05) decrease in the mean total sialic acid (TSA) levels on days 7 and 14 after treatment as compared to the pre-treatment levels in all the three groups. Mean somatic cell count (SCC), standard plate count (SPC) and electrical conductivity (EC) values decreased significantly on days 4, 7 and 14 as compared to that of the day 1. It was concluded that therapeutic intervention with parenteral enrofloxacin and intramammary infusion of proteolytic enzymes improved the udder health of dairy cows affected with clinicalmastitis. Keywords: Antibiotic, Clinical mastitis, Multienzyme Mastitis or udder inflammationis a worldwide problem that adversely affects the animal health and quality of milk [11]. Contagious bovine mastitis is mostly caused by Staphylococcus aureus generally manifesting as a chronic recurrent infection which exhibits resistance to antibiotic therapy [17]. The ability of Staphylococci to form biofilms in the infected tissues obstructs the treatment of recurrent infections [9]. The enzymes can be used for degradation of biofilm [6]. A proteolytic drug with fibrinolytic properties facilitates penetration of antibiotics into the infected and inflamed tissuein cases of acute bovine mastitis to thereby accelerating recovery of the gland. The present study evaluated the efficacy of multienzyme therapy as an adjunctor alternative to antibiotic therapy in mastitis affected cows. MATERIALS AND METHODS Twenty one HF cross bred dairy cows in 2 nd to 5 th parity were selected on a dairy farm and diagnosed for clinical mastitis on the basis of clinical signs, physical examination and California mastitis test (CMT). On microbiological examination milk samples from the affected quarters revealed the presence of Staphylococcus aureus.An on-farm trial was carried out in these mastitis affected animals with selected antibiotic to which the bacterial isolates showed maximum sensitivity i.e., enrofloxacin alone and along with the multienzyme therapy (chymotrypsin, trypsin and papain) by randomly allocating animals to one of the following three treatment groups: