Research Article Efficient Shoot Organogenesis Using Leaf Disc and Nodal Explants of Passion Fruit (Passiflora edulis Sims) and Genetic Fidelity Assessment Using Sequence-Related Amplified Polymorphism (SRAP) Markers Lydia K. Asande , 1,2 Omwoyo Ombori, 1 Evans N. Nyaboga, 2 and Richard O. Oduor 3 1 Department of Plant Sciences, Kenyatta University, P.O. Box 43844–00100, Nairobi, Kenya 2 Department of Biochemistry, University of Nairobi, P.O. Box 30197–00100, Nairobi, Kenya 3 Department of Biochemistry Microbiology and Biotechnology, Kenyatta University, P. O. Box 43844–00100, Nairobi, Kenya Correspondence should be addressed to Lydia K. Asande; lydasande@yahoo.com Received 12 March 2020; Revised 14 June 2020; Accepted 19 June 2020; Published 10 July 2020 Academic Editor: Wei Wu Copyright © 2020 Lydia K. Asande et al. is is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Passion fruit (Passiflora edulis (Sims)) is currently ranked third among fruit exports from Kenya and has great potential since the demand for both fresh fruit and processed juice is on a continuous increase. Passion fruit production in Kenya is constrained by a lack of healthy, clean planting material, poor seed viability, and low germination rates. To address this, the present study reports an in vitro plant regeneration protocol for passion fruit using leaf disc and nodal explants and genetic fidelity analysis of the regenerated plants. e highest number of shoot regeneration was obtained on Murashige and Skoog (MS) medium supplemented with 2 mg·L -1 6-Benzyl amino purine (BAP) (shoot induction medium). e multiplication of shoots was optimum in MS medium supplemented with 3 mg·L -1 BAP. To eliminate the requirement of an additional step of in vitro rooting, exogenous application of putrescine induced the formation and development of roots on nodal explants. Genetic fidelity analysis of the in vitro regenerated and macropropagated plants with that of the mother plant was carried out by sequence-related amplified polymorphism (SRAP) markers, and monomorphic banding profile for 80% of the regenerants confirmed the genetic uniformity of the in vitro regenerated and macropropagated plants. e in vitro regeneration system developed can be utilized for mass clonal propagation for the economic commercial exploitation of this important tropical fruit. 1.Introduction Passion fruit (Passiflora edulis Sims) is an economically important perennial fruit crop in many tropical and sub- tropical countries, mainly grown for its edible fruit and ornamental and medicinal use [1–3]. It is a significant component of the horticulture industry which sustains millions of livelihoods in Kenya through local and export markets [4]. Moreover, the crop has great commercial po- tential in Kenya since the demand for both fresh fruit and processed juice is on the increase besides the expanding export markets [5]. e production of passion fruit in Kenya has remained low at an average of 8 ton·ha -1 compared to a potential of 24 ton·ha -1 mainly due to pests and diseases and inadequate clean planting materials [4, 6]. ese constraints have led to the reduction of the lifespan of the plants in the field from 7 years to an average of 1 to 2 years [4, 7]. Among the diseases limiting passion fruit production is woodiness disease (PWD)-complex which is mainly transmitted by aphids. is is a serious disease in all passion fruit production areas in Kenya, affecting both the purple and the yellow forms [8, 9]. e disease has been reported to cause up to 100% loss in fruit yields in Kenya [10]. e mode of propagation entails the use of seed and grafting which has led to the build-up of diseases especially of the woodiness virus complex resulting in total yield losses and lack of clean planting materials. Hindawi International Journal of Agronomy Volume 2020, Article ID 3205710, 10 pages https://doi.org/10.1155/2020/3205710