Vol. 13 | No. 4 |2215-2222| October - December | 2020 ISSN: 0974-1496 | e-ISSN: 0976-0083 | CODEN: RJCABP http://www.rasayanjournal.com http://www.rasayanjournal.co.in Rasayan J. Chem., 13(4), 2215-2222(2020) http://dx.doi.org/10.31788/ RJC.2020.1345818 EVALUATION OF ANTIBACTERIAL AND ANTIOXIDANT ACTIVITIES OF XANTHONE ISOLATED FROM Orophea corymbosa LEAF H. P. C. A. Cane 1 , N. Saidi 2 , M. Mustanir 2 , D. Darusman 3 , R. Idroes 2,4 and M. Musman 5,* 1 Graduate School of Mathematics and Applied Sciences, Graduate Program/Universitas Syiah Kuala, Banda Aceh-23111, (Aceh) Indonesia 2 Department of Chemistry, Faculty of Mathematics and Natural Science/Universitas Syiah Kuala, Banda Aceh-23111, (Aceh) Indonesia 3 Department of Soil Science, Faculty of Agriculture/Universitas Syiah Kuala, Banda Aceh- 23111, (Aceh) Indonesia 4 Department of Pharmacy, Faculty of Mathematics and Natural Science/Universitas Syiah Kuala, Banda Aceh-23111, (Aceh) Indonesia 5 Department of Chemistry Education, Faculty of Education and Teacher Training/Universitas Syiah Kuala, Banda Aceh-23111, (Aceh) Indonesia *E-mail: musrimusman@unsyiah.ac.id ABSTRACT The pharmacological properties (antibacterial and antioxidant) of the ethyl acetate extract from the leaves of Orophea corymbosa Blume Miq. the plant was evaluated and continued for obtaining bioactive compounds through the isolation process. A pure isolate from ethyl acetate extract was analyzed of structure compound using NMR Spectra, FTIR, UV-Vis, and MS (GCMS). An isolated compound was considered to be xanthone derived compound, namely 1- hydroxy-2,3-dimethoxyxanthone. Its activities against Gram-negative and positive bacteria were examined with bioindicator Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923, respectively, where the results were then compared to cephazolin; and antioxidant activity with indicator free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) compared to ascorbic acid. The xanthone compound has good activity as antibacterial and antioxidant. The plant has administrated the potential for handling bacteria and free radicals in this study. Keywords: Annonaceae, Antibacterial, Antioxidant, Escherichia coli, Staphylococcus aureus, Xanthone. © RASĀYAN. All rights reserved INTRODUCTION The application of natural products as medical objects is still in high demand. The natural product is a favorable choice with fewer side effects, safer, 1 easy to find, and green chemistry. 2 The discovery of natural products, especially in a tropical and subtropical country, can be simply found in plants. The plants were widely reported to have medicinal property from their metabolites, 3 which are produced by plant 4 or endophytic bacteria 5 as self-defense against pathogens. 6,7 The metabolites have a lot of medical activities as an antibacterial, 8 antioxidant, 9 antimicrobial, 10 antifungal, 11 wound healing, 12 and antibiofilm 13 agents. The use of plant parts such as a leaf, 14 roots, 15 and even fruit 16 has been traditionally done by indigenous peoples since long time ago. Furthermore, to obtain the metabolite can be simply done by extraction 17 . The Annonaceae family is widespread in diverse habitats (forests, shrubs and grasslands) in both tropical and subtropical areas and is abundant in rainforest areas around the world. 18 Many genera from the family were found to have medicinal properties against ulcers, fever, infection, abdominal pains, bronchitis, asthma and wounds. 19 Moreover, they also have pharmacological activities like antifungal, antibacterial, antimalarial, antioxidant and anticancer agents. 20,21 Bioactive compounds from plants (i.e xanthone) play an important role in terms of medicine. 22,23 Xanthone compounds are compounds with a three-ring