Vol.9 (2019) No. 5 ISSN: 2088-5334 Characterization of Probiotic Bacterial Candidates from Jatinangor- Indonesia Breast Milk In-In Hanidah # , Indira Lanti Kaya Putri # , Wendry Setiyadi Putranto * , Bambang Nurhadi # , Debby Moody Sumanti # # Food Industrial Technology Department, Faculty of Agricultural Industrial Technology, Universitas Padjadjaran, 45363 Sumedang, Indonesia. Email: inin@unpad.ac.id * Laboratory of Animal Products Technology, Faculty of Animal Husbandry, Universitas Padjadjaran, 45363 Sumedang, Indonesia. Abstract— Breast milk is an important nutrient for neonates in body’s nutritional needs and immune system formation. Some research show breast milk generally contains Bifidobacteria and probiotic bacteria species of Lactobacillus. Microbiota in the breast milk from every area is diverse, bacteria isolated from healthy mothers’ breast milk from Taiwan and six regions of China (Central, East, North, Northeast, South, and Southwest China). It shows Streptococcaceae (24.4%), Pseudomonadaceae (14.0%), Staphylococcaceae (12.2%), Lactobacillaceae (6.2%), and Oxalobacteraceae (4.8%). Germany or Austria provided the breast milk from 160 women contain L. salivarius (35.00%), L. fermentum (25.00%), L. gasseri (21.88%), and Bifidobacterial species (13.75%). Spanish provided Staphylococcus, Pseudomonas, Streptococcus, and Acinetobacter dominated the breast milk from 21 healthy mother. In this study, isolation and characterization of candidates for probiotic bacteria from fifteen breast milk samples from Sumedang – Indonesia. The main purpose of this study was to isolate and identify probiotic bacteria that are resistant pH 2 and tolerance of 0.3% bile concentration. The results showed that were twelve isolate candidate probiotic bacteria able to grow on pH 2 media for 2 hours and tolerance of 0.3% bile concentration. Only two of them had the best growth and potential probiotic candidates. Base on Biochemical identification using the Vitek 2.0 Card type: ANC testing instrument 00001658F4A9 (12903), there are Staphylococcus hominis (8.3%) and Lactobacillus plantarum (8.3%). In the future, S. hominis will be probiotic bacteria that can be applied as functional food. Keywords— breast milk; probiotic; Staphylococcus hominis; Lactobacillus plantarum; functional food. I. INTRODUCTION Breast milk is a perfect nutrient for infants [1] and potentially probiotic bacteria to the infant gut [2]. It is among others: immunology, biochemical component (proteins, lipids, carbohydrates, biological active), and cellular component that are very potential for the newborn’s immune system from various infections [3]. These components are very important for infants and it transfers microflora originated in breast milk [4]. Breast milk is also proven to be a source of commensal and probiotic bacteria such as Staphylococcus, Streptococcus, and Lactic Acid Bacteria (LAB) [5]. Some research show breast milk generally contains Bifidobatreria [6] and probiotic bacteria species of Lactobacillus [7]. Microbiota in the breast milk from every area is diverse, bacteria isolated from healthy mothers’ breast milk from Taiwan and six regions of China (Central, East, North, Northeast, South, and Southwest China). It shows Streptococcaceae (24.4%), Pseudomonadaceae (14.0%), Staphylococcaceae (12.2%), Lactobacillaceae (6.2%), and Oxalobacteraceae (4.8%) [8]. Germany or Austria provided the breast milk from 160 women contain L. salivarius (35.00%), L. fermentum (25.00%) and L. gasseri (21.88%), and Bifidobacterial species (13.75%) [6]. Spanish provided the breast milk from 21 healthy mother was dominated by Staphylococcus, Pseudomonas, Streptococcus, and Acinetobacter[9]. In another Spanish, the healthy core microbiome included the genera Staphylococcus, Streptococcus, Bacteroides, Faecalibacterium, Ruminococcus, Lactobacillus, and Propionibacterium [10]. Although probiotic strains can be isolated from many sources, but for human applications the main criteria is being human origin [11]. Geographical location can directly affect the microbiota and fatty acid content in breast milk[8]. Milk bacterial communities were generally complex and showed individual specific profiles [9]. 1649