37 ORIGINAL ARTICLE © Turk J Pharm Sci, Published by Galenos Publishing House. Turk J Pharm Sci 2019;16(1):37-42 DOI: 10.4274/tjps.18894 ÖZ Amaç: Bazı mantar türlerinin nematofagöz ve antiprotozoan olduğu bildirilmiştir. Bu çalışmada, yenilebilir mantar Pleurotus ostreatus n-hekzan ekstresinin antiplazmodiyal ve sitotoksik etkileri araştılmış ve ekstreden bir sterol izolasyonu yapılmıştır. Gereç ve Yöntemler: Antiplazmodiyal ve sitotoksisite deneyleri, sırasıyla, plasmodium laktat dehidrojenaz analizi ve insan HeLa servikal hücre hatları kullanılarak in vitro gerçekleştirilmiştir. n-Hekzan ekstresinden izole edilen bileşiğin yapısı, spektroskopik teknikler kullanılarak aydınlatılmıştır. Bulgular: n-Hekzan ekstresi (verim: %0.93 a/a) parazit inhibisyonunda doza bağlı antiplazmodiyal aktivite gösterdi: klorokin (IC 50 =0.016 μg/mL) > n-hekzan ekstresi (IC 50 =25.18 μg/mL). Ayrıca referans ilaç emetine kıyasla (IC 50 =0.013 μg/mL) hafif sitotoksisite (IC 50 >100 μg/mL; seçicilik indeksi >4) gösterdi. Bilinen sterol bileşiği ergostan-5,7,22-trien-3-ol izole edildi ve yapısı tayin edildi. Sonuç: P. ostreatus’un antiplazmodiyal aktivitesini ilk kez rapor eden bu çalışma, sıtma tedavisindeki nutrasötik potansiyelini ortaya koymuştur. Anahtar kelimeler: Pleurotus ostreatus, nutrasötik, malarya, sitotoksisite, ergosterol *Correspondence: E-mail: ozadheoghene.afieroho@uniport.edu.ng, Phone: +2348063432417 ORCID-ID: orcid.org/0000-0002-5847-161X Received: 03.12.2017, Accepted: 18.01.2018 Objectives: Several mushrooms species have been reported to be nematophagous and antiprotozoan. This study reported the antiplasmodial and cytotoxic properties of the n-hexane extract from the edible mushroom Pleurotus ostreatus and the isolation of a sterol from the extract. Materials and Methods: Antiplasmodial and cytotoxicity assays were done in vitro using the plasmodium lactate dehydrogenase assay and human HeLa cervical cell lines, respectively. The structure of the isolated compound from the n-hexane extract was elucidated using spectroscopic techniques. Results: The n-hexane extract (yield: 0.93% w/w) showed dose dependent antiplasmodial activity with the trend in parasite inhibition of: chloroquine (IC 50 =0.016 μg/mL) > n-hexane extract (IC 50 =25.18 μg/mL). It also showed mild cytotoxicity (IC 50 >100 μg/mL; selectivity index >4) compared to the reference drug emetine (IC 50 =0.013 μg/mL). The known sterol, ergostan-5,7,22-trien-3-ol, was isolated and characterized from the extract. Conclusion: This study reporting for the first time the antiplasmodial activity of P. ostreatus revealed its nutraceutical potential in the management of malaria. Key words: Pleurotus ostreatus, nutraceuticals, malaria, cytotoxicity, ergosterol ABSTRACT 1 University of Port Harcourt, Faculty of Pharmaceutical Sciences, Department of Pharmacognosy and Phytotherapy, Port Harcourt, Nigeria 2 Rhodes University, Faculty of Science, Department of Chemistry, Grahamstown, South Africa 3 University of Port Harcourt, Faculty of Agriculture, Department of Crop and Soil Sciences, Port Harcourt, Nigeria 4 Rhodes University, Faculty of Science, Department of Biochemistry and Microbiology, Grahamstown, South Africa Ozadheoghene Eriarie AFIEROHO 1* , Xavier Siwe NOUNDOU 2 , Chiazor P. ONYIA 1 , Osamuyi H. FESTUS 1 , Elizabeth C. CHUKWU 1 , Olutayo M. ADEDOKUN 3 , Michelle ISAACS 4 , Heinrich C. HOPPE 4 , Rui WM. KRAUSE 2 , Kio A. ABO 1 Pleurotus ostreatus (Jacq. ex. Fr) P. Kumm. n-Hekzan Ekstresinin Antiplazmodiyal Etkisi Antiplasmodial Activity of the n-Hexane Extract from Pleurotus ostreatus (Jacq. ex. Fr) P. Kumm.