Journal of Neuroirnmunotogy, 3 (1982) 43-62 ElsevierBiomedical Press : Human Fetal Brain Antigen Expression Common to Tumors of Neuroectodermal Tissue Origin Gliomas, Neuroblastomas, and Melanomas Carol J. Wikstrand I, Mario A. Bourdon 1, Charles N. Pegram i and Darell D. Bigner 1.2., s Department of Pathology, and 2 Department o t Surgery, Duke University Mea~cal Center, Durham, NC 27710 (U.S.A.) (Receive~130 October,1981) (Revised,received17 February,1982) (Accepted 17February, 1982) Sunm, ary The antigenic relationship between human tumors of neurcectodermal origin and fetal brain were further investigated by characterization of two hybridoma antibod- ies derived from a fusion of P3.NSI/I-Ag 4-1 (NSI) myeloma cells and splenocytes hyperimmunized to second triraester human fetal brain homogenate. Monoclonal antibodies (MAs) 1H8cl 2 and IHScl 3 were analyzed by cell surface radioim- munoassay (CS,RIA), quantitative absorption, indirect immunofiuorescence, and peroxidase-antiperoxidase (PAP) immuL, ohistology. MA IH8cl 3 is the more broadly reactive, binding to 9/14 glioblastoma (GBM), 2/3 neuroblastoma, !/2 lndanoma, and 1 medulloblastoma cell fine(s) by CS-RIA analysis, and to 12/15 GBM, fetal brain, spleen, and fiver, and adult spleen by PAP a~alysis. MA lHScl 2 is more reslricted, binding to 7/14 GBM, 2/3 neuroblastoma, I medulloblastoma, and 2/3 fetal skin fibroblast cell fine(s) by CS-RIA, and to 9/' 15 GBM and fetal brain and spleen by PAP analysis. Control non-central nervou~ system tumors and normal adult tissue including brain, thymus, lymph node, liver, kidney, lung, skin. and p~ncreas, were unreactive with both l HScl 2 and l H8cl 3 by CS-RIA, PAP, and * To whomrequests for reprintsshouldt~eaddressed. Abbreo/~/om: BR----bindi~ ratio; eXl~pm/bkgdepm; BR~'3.0 considered sigaificantly pmitive~CS- RIA=cell surfsce radioimmunoaumy; DPBS=Dulbeo~'s phosphate-bufferedsaline; FCS--fetal calf mum; GBM=81ioblmtoma multifonne; HAT=selectionmedium(Littlefield 1964); H=hypoxmthime; A:=anmtoptm~ai T=thymidine; HBSS=Hank's balanced salt solution; MA--monoclomal amibody; NSI =mydmm cell1~ P3-NSi/I-A 8 4-1; PAP---peroxidase--antipemxidase immunohistoehemi~ amy: ZOG=gx~..~ion me, odium, mpplmuemecr with pntamicin, 81utamin~and 10-.-20~ FCS. 016S-$728/82/0000-000U/S02.75 ~ 1982ElsevierBiomedicalPress