~ 12 ~ International Journal of Plant Pathology and Microbiology 2021; 1(1): 12-15 E-ISSN: xxxx-xxxx P-ISSN: xxxx-xxxx IJPPM 2021; 1(1): 12-15 Received: 12-11-2020 Accepted: 17-12-2020 Md. Anwar Sagar School of Agriculture, Uttaranchal University, Dehradun, Uttarakhand, India Rajendra Prasad School of Agriculture, Uttaranchal University, Dehradun, Uttarakhand, India Sandeep Kumar School of Agriculture, Uttaranchal University, Dehradun, Uttarakhand, India Mahipal Singh School of Agriculture, Uttaranchal University, Dehradun, Uttarakhand, India Correspondence School of Agriculture, Uttaranchal University, Dehradun, Uttarakhand, India In vitro efficacy of some fungicides against Fusarium oxysporum f. sp. pisi a causal agent of wilt disease of Pea (Pisum sativum L.) Md. Anwar Sagar, Rajendra Prasad, Sandeep Kumar and Mahipal Singh Abstract In the present study, some fungicides were tested for their ability to inhabit or cidal effect against a plant pathogen Fusarium oxysporum f. sp. pisi causes wilt disease of Pea (Pisum sativum L.) cultivated during the cool season in subtropical and temperate countries. The experiment was carried out by poisoned food technique in which different doses of fungicides were mixed in Potato Dextrose Medium (PDA) and growth inhibition of fungal pathogen was observed. The fungicides tested were Carbendazim 50% WP, Tebuconazole 10% + Sulphur 65% WG, Propineb 70% WP, Metiram 70% WG at four different concentrations (25ppm, 50ppm, 100ppm and 200ppm). The treatments were arranged in completely Randomized Design was followed (CRD) with three replications of each treatment and plate having no treatments served as control. Growth was observed after 3 days, 6 days and 9 days intervals after the inoculation. Maximum inhibition of growth of F. oxysporum f.sp. pisi was reported with the all doses used of fungicide combination of Tebuconazole10% + Sulphur 65%WG and then followed by higher dose (200ppm) of Carbendazim 50%WP. The fungicides Propined 70%WP and Metiram70%WG were found very less effective against fungal pathogen. The higher concentration these two fungicides (200ppm) used, inhibit 33.33% and 25.25.19%, respectively. The study revealed that combination of Tebuconazole 10% + Sulphur 65%WG can be explored to control the wilt disease of pea crops in the field. Keywords: Fungicides, mycelium growth, percent inhibition and poison food technique Introduction Pea (Pisum sativum L.) is a winter season important annual autogamy (2n=14) pulse crop belongs to legume family (McKay et al., 2003). Pea improves the soil fertility by (nitrogen fixation by Rhizobium leguminosarum) present in root nodules and thus reduced quantity of fertilizer required. It is cultivated in wide soil kind starting from light-weight sandy loams to serious clayey with optimum pH scale 5.5 to 6.5 (FAO, 2012; CSA, 2015-16). Pea grains are rich in vitamins, dietary fibers, minerals, fats 1.4%, Proteins 27.8% and carbohydrates up to 42.65% (Tzitzikas et al., 2006) [9] . World pea production was over ten million in tones in 2009. Major producers of peas are Russian Federation, Canada, Asian nation, China and USA. India is that the second largest producer of pea within the world and Pea occupies 459-thousand-hectare space in Asian nation and shares 21% production of the world. Uttarakhand state shares 1.72% and production is 93.40 tones (According to National Horticulture Board (NHB) 2017-2018 data) it's utilized in contemporary state additionally as processed form. In fresh state, primarily snow pea and sugar snap are exported because of their high demand in international market. Pea are grown on large areas in India but Uttarakhand production or yield is less as compared to other states. One of the main reasons is diseases and insect pests. Some destructive fungi are associated with pea like Ascochta pisi, Cladosporium piscicola, Sclerotina sclerotiorum and Fusarium oxysporum. Among them, F. oxysporum is considered most destructive soil borne pathogen. Pea wilt caused by Fusarium oxysporum f. sp. pisi. In past few years, many manmade chemicals were introduced against Phytopathogen. The mishandling not solely crystal rectifier to the event of chemical resistance in pathogens however also established unsafe to each animals and human health additionally as impart negative impact on plant growth (Okigbo et al., 2004) [7] . In recent years, Wilt of Pea has become serious in several pea growing areas in India, up to now not a lot of analysis has been done.