IOSR Journal Of Pharmacy And Biological Sciences (IOSR-JPBS) e-ISSN:2278-3008, p-ISSN:2319-7676. Volume 16, Issue 4 Ser. I (Jul. – Aug. 2021), PP 45-49 www.Iosrjournals.Org DOI: 10.9790/3008-1604014549 www.iosrjournals.org 45 | Page Formulation, Evaluation of Paper Mask Essence from Bovine Colostrum Kefir and Determination of Its Biological Activity Fenti Fatmawati 1* , Yanni Dhiani Mardhiani 1 , Garnadi Jafar 1 , ED Yunisa Mega Pasha 1 , Rahmayanti Nurraeni 1 , Ilma Naila Saidah 1 , Angga Putra Pribadi 1 1 (Faculty of Pharmacy, Bhakti Kencana University, Indonesia) Abstract: Background: The use of colostrum in Indonesia has not been widely used. At the farmer level, if the early lactation cow production is too high, colostrum is sometimes discarded because it is considered milk that does not meet the requirements and will be rejected after being stored, this is very detrimental to smallholders. Bovine colostrum and kefir both have biological benefits for the body. Therefore, in this study, an essence for a paper mask was made from bovine colostrum kefir. The aim of this study was to formulate and evaluate of paper mask essence made from Bovine Colostrum kefir and to determine its biological activity. Materials and Methods The material used was Bovine colostrum kefir. This study using the titration method to calculate the levels of lactic acid, diffusion method for antibacterial activity, DPPH for antioxidant activity, formulation evaluation, and data were analyzed using SPSS. Results: Formula F1 can be said to be good because it meets all the evaluation criteria for the preparation. Homogeneous preparations with pH 4.6-4.64, viscosity 264-232 cps, non-irritating to the skin, and stable for 4 weeks of storage. By using the non-parametric Kruskal Wallis test to see the effects of pH value, viscosity, and the lactic acid content from the formulas caused by the storage time , obtained a value of p<0,05 means there were significant differences between formulations. Contains antioxidants with an IC50 value of 76,689 ppm and has moderate inhibitory activity against Staphylococcus epidermis bacteria. Conclusion: Based on the results of the evaluation shows that BC kefir can be formulated into paper mask essence preparations and have an antioxidant and antibacterial activity Key Word: Antibacterial ; Antioxidant; Bovine colostrum; Essence; Kefir --------------------------------------------------------------------------------------------------------------------------------------- Date of Submission: 08-07-2021 Date of Acceptance: 23-07-2021 --------------------------------------------------------------------------------------------------------------------------------------- I. Introduction Bovine colostrum (BC) is a nutrient-rich biological liquid produced by cows after parturition 1 . Difference from milk, bovine colostrum contains more lactalbumin and lactoprotein. It also contains protein, immunoglobulin, lactoferrin, carbohydrates, oligosaccharides, fats, vitamins, minerals, anti-microbial compounds, and growth factors 2 . Colostrum is also used as a topical healing agent that contains growth factors that are important for healing new skin. Kefir is a fermented milk product using a lactic acid microbial starter (bacteria and yeast). Kefir has antimicrobial, antitumor, anti-inflammatory, and antioxidant activity 3 . Kefir was considered good for skin health because it contains lactic acid which functions to treat the skin, for example as an antibacterial agent, helps in the regeneration of dead skin cells, and brightens the skin. The aim of this study was to formulate and evaluate of paper mask essence made from BC kefir and to determine its antioxidant and antibacterial activity. II. Material And Methods The material used was Bovine colostrum (BC). Other ingredients used were Staphylococcus epidermis bacteria, xanthan gum, glycerin, propylene glycol, PEG-40 Hydrogenated Castor Oil, DMDM Hydantoin, aqua dest, PP, Nutrient agar, 96% ethanol, methanol, DPPH, ascorbic acid, aqua DM, NaOH, oxalate acid. Procedure methodology Essence formulation was made with BC kefir concentration of 5% in the F1 formula, 10% in the F2 formulation, and 15% in the F3 formulation. F0 was used as a comparison made without using BC kefir. Xanthan gum was dissolved with hot distilled water, then stirred until it swelled using a magnetic stirrer for 1 hour at a speed of 250 rpm. BC kefir was added little by little stir until homogeneous. Propylene glycol and glycerin were homogenized (mixture A). PEG-40 hydrogenated castor oil was dissolved using distilled water,