442 ISSN: 2348-1900 Plant Science Today http://www.plantsciencetoday.online Research Article In vitro clonal propagation, organogenesis and somatic embryogenesis in Bacopa monnieri (L.) Wettst Dipu Samanta 1 , Bidisha Mallick 2 & Debleena Roy 2* 1 Department of Botany, Dr. Kanailal Bhattacharyya College, Ramrajatala, Howrah 711 104, India 2 Department of Botany, Lady Brabourne College, P-1/2, Surahwardy Avenue, Kolkata 700 017, India Artic le history Received: 24 June 2019 Accepted: 02 August 2019 Published: 01 October 2019 Guest Editor Dr Nishikant Wase Publisher Horizon e-Publishing Group *Correspondence Debleena Roy debleenaroy@rediffmail.com Abstract Bacopa monnieri (L.) Wettst is a well-known medicinal herb in the Ayurveda. It is also used as laxative and curative for ulcers, inflammation, anaemia, scabies, leucoderma, asthma and epilepsy, enlargement of spleen, leprosy and others. In vitro propagation and regeneration through somatic embryogenesis of B. monnieri has played an important role in the production of healthy, disease-free plants with desirable traits. In B. monnieri, there are few reports which indicate rapid regeneration and somatic embryogenesis. For in vitro clonal propagation, the highest shoot formation was obtained when BAP 2 mg/ l used. The best response for rooting was obtained in IAA 1.0 mg/ l. The recorded survival rate of the plants was 70%. Plants were without any detectable phenotypic variations. Cytological study indicated that the chromosome number remain same (2n= 64) in in vitro and in vivo roots. A rapid, simple and efficient protocol for plantlet regeneration was achieved through embryogenic callus from leaf explants of B. monnieri. Callus induction and embryogenesis were significantly affected by presence/absence and type and concentration of growth regulators. Best organogenic callus induction was obtained in MS medium supplemented with BAP 5mg/ l. For induction of somatic embryogenesis, auxin (2, 4-D 1 mg/ l) was used in the culture medium subsequently in basal media for embryo maturation. Kn 0.2 mg/ l was the best for production of plantlet from embryo. Thus, this can be an easiest protocol for stable clonal propagation and plant regeneration through somatic embryogenesis in B. monnieri. The protocol used here for propagation and regeneration is much easier, low cost and reliable. Keywords: Bacopa monnieri; in vitro; Organogenesis; Rooting; Shoot Bud Multiplication; Somatic Embryogenesis Citation: Samanta D, Mallick B, Roy D. In vitro clonal propagation, organogenesis and somatic embryogenesis in Bacopa monnieri (L.) Wettst. Plant Science Today 2019;6(4):442-449. https:// doi.org/10.14719/pst.2019.6.4.600 Copyright: © Samanta et al (2019). This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited (https://creativecommons.org/licenses/by/4.0/ ). Indexing: Plant Science Today is covered by Scopus, Web of Science, BIOSIS Previews, ESCI, CAS, AGRIS, CABI, Google Scholar, etc. Full list at http://www.plantsciencetoday.online Introduction Bacopa monnieri (L.) Wettst. (Scrophulariaceae) is a well-known medicinal herb in the Ayurveda. The International Union for Conservation of Natural and National Resources has a long time ago listed Bacopa monnieri as a threatened species but at present Bacopa monnieri comes under Least Concern Category (1). The plant is commonly known as “Brahmi” and found as spreading herbs. Horizon e-Publishing Group ISSN: 2348-1900 Plant Science Today (2019) 6(4): 442-449 https://doi.org/10.14719/pst.2019.6.4.600