1 .Supporting Information (SI) for Live Imaging of Cell Membrane-Localized MT1-MMP Activity on a Microfluidic Chip Nan Li a1 , Weifei Zhang a1 , Ling Lin b ,*, Ziyi He a , Mashooq Khan a , and Jin-Ming Lin a ,* a Beijing Key Laboratory of Microanalytical Methods and Instrumentation, MOE Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Department of Chemistry, Tsinghua University, Beijing, 100084, China. E-mail: jmlin@mail.tsinghua.edu.cn Tel: +86-10-62792343 b CAS Key Laboratory of Standardization and Measurement for Nanotechnology, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing 100190, P.R. China. E-mail: linling@nanoctr.cn Experimental sections 1. Reagents and apparatus SU-8 2050 Negative photoresist and developer were purchased from Microchem Corporation (Newton, MA, USA). PDMS prepolymers and initiators were purchased from Dow Corning (Midland, MI, USA). 5-TAMRA-βAlaSLAPLGLQRRK-5-FAM-C-NH2 was synthesized from Bankpeptide biological technology co., LTD (Hefei，China). Recombinant human MT1- MMP protein, rhFurin protein, transforming growth factor beta 1 (TGF-β1) and vascular endothelial growth factor was purchased from R&D Systems (Minneapolis, MN, USA). Matrigel was bought from Corning, America. Marimastat was obtained from MedChem Express (Shanghai, China). CellTracker Red CMTPX dye was obtained from ThermoFisher Scientific (Shanghai, China). Hoechst was purchased from the Sigma (Shanghai, China). N-(4- Maleimidobutyryloxy) succinimide (NHS-Maleimido) and cell counting kit-8 (CCK-8) was bought from Dojindo Corporation (Tokyo, Japan). Fetal bovine serum (FBS) was from Hyclone Electronic Supplementary Material (ESI) for ChemComm. This journal is © The Royal Society of Chemistry 2018