Research Article Occurrence, Morphometric, and Molecular Investigation of Cattle and Buffalo Liver Adult Fluke in Peninsular Malaysia Main Abattoirs J. Nur Alia Diyana, 1 M. I. Nur Mahiza , 1 H. Latiffah, 1 S. H. Nur Fazila, 1 I. H. Lokman, 1 H. Noor Hazfalinda , 2 P. Chandrawathani, 3 E. B. Ibitoye, 4 and K. Juriah 5 1 Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia 2 Faculty of Health Sciences, Universiti Kebangsaan Malaysia, 43600, UKM, Bangi, Selangor, Malaysia 3 Research and Innovation, Department of Veterinary Services, 62624 Putrajaya, Malaysia 4 Department of Theriogenology and Animal Production, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, Nigeria 5 Faculty of Agriculture and Food, Universiti Putra Malaysia Bintulu Sarawak, 97008 Sarawak, Malaysia Correspondence should be addressed to M. I. Nur Mahiza; nurmahiza@upm.edu.my Received 20 February 2020; Revised 5 May 2020; Accepted 23 May 2020; Published 12 June 2020 Academic Editor: Bernard Marchand Copyright © 2020 J. Nur Alia Diyana et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Fascioliasis is a parasitic disease of human and animal caused by Fasciola gigantica (F. gigantica) and Fasciola hepatica (F. hepatica). More than 700 million of grazing animals and over 180 million human population are at the risk of fascioliasis. This study was conducted in Banting, Ipoh, Shah Alam, and Taiping abattoirs within Peninsular Malaysia to determine the occurrence and identify the species of liver flukes, causing liver condemnation in cattle and buffaloes. Within a study period from January to December 2018, a total of 25 condemned bovine livers were collected from Banting, Ipoh, Shah Alam, and Taiping abattoirs and analyzed. Taiping abattoir had the highest occurrence of fascioliasis [14/1014 (1.38%)], and Shah Alam abattoir had the least occurrence [1/3377 (0.03%)]. From all the sampled livers, the average number of adult flukes recorded ranged from 1 to 83. A total of 440 adult flukes were studied morphometrically, using parameters such as body length (BL), body width (BW), cone length (CL), cone width (CL), body area (BA), body perimeter (BP), the distance between the ventral sucker and posterior end of body (VS-P), BL/BW ratio, BL/V-SP, and body roundness (BR). Furthermore, molecular analysis was conducted using PCR- RFLP methods to distinguish between F. gigantica and F. hepatica involving ITS1 primer and RsaI restriction enzyme. RFLP pattern with RsaI produced a consistent pattern of 360,100 and 60 bp fragments in F. hepatica, whereas F. gigantica worms had a profile of 360,170, and 60 bp in size. The morphometric and molecular analysis results indicated that cattle and buffaloes slaughtered at Banting, Shah Alam, Taiping, and Ipoh abattoirs were infected with F. gigantica. 1. Introduction Fascioliasis is common among grazing animals in most parts of Malaysia, and its prevalence can reach up to 50% in some provinces [1]. F. gigantica and F. hepatica are the two aetiologic species of fascioliasis [2]. F. hepatica is widely distributed worldwide, but F. gigantica is distrib- uted primarily in the tropical and subtropical regions [3]. It is essential to differentiate between species of Fasciola. Although intermediate host for both species (F. hepatica and F. gigantica) vary in terms of biological and ecological characteristics, intermediate forms between the two Fas- ciola species have been extensively reported in shared hosts in Korea [4], Japan [5], Vietnam [6], China [7], and Iran [8] [9]. In some countries in Asia, F. hepatica and F. gigantica coexist, and the intermediate fasciolid Hindawi Journal of Parasitology Research Volume 2020, Article ID 5436846, 6 pages https://doi.org/10.1155/2020/5436846