Research Article Effect of In Vitro Transcorneal Approach of Aceclofenac Eye Drops through Excised Goat, Sheep, and Buffalo Corneas Vivek Dave, Sarvesh Paliwal, Sachdev Yadav, and Swapnil Sharma Department of Pharmacy, Banasthali University, Banasthali 304022, India Correspondence should be addressed to Vivek Dave; vivekdave1984@gmail.com Received 4 July 2014; Revised 1 September 2014; Accepted 11 September 2014 Academic Editor: Paul Danckwerts Copyright © 2015 Vivek Dave et al. Tis is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Te current study involves the evaluation of factors that infuence the transcorneal permeation of aqueous drops of aceclofenac ophthalmic formulation through freshly excised goat, sheep, and bufalo corneas. Aceclofenac formulation with diferent concentrations 0.1–0.5%(w/v) and with diferent pH and diferent preservatives, was taken into account. Te amount of drug permeated from diferent formulations was estimated using an Franz difusion cell. A linear increase in drug permeation was observed with increase in pH (5.5 to 7.4). Te apparent permeability coefcient was found to be maximum 15.01±0.45 on goat cornea and maximum transport of aceclofenac was observed at physiological pH of tears (i.e., 7). Te results advocate that aceclofenac 0.5%(w/v) ophthalmic solution (pH 7.0) containing BAK (0.01%) provides maximum in vitro ocular permeability through goat, sheep, and bufalo corneas. 1. Introduction Te feld of ocular drug delivery is one of the interesting and challenging endeavors facing the pharmaceutical scientist. Te cornea is a transparent tissue in the eye that is responsible for the refraction of incoming light and is a multilayered tissue made up of three major cell layers: the epithelium, the stroma, and the endothelium [1]. A corneal epithelium is a stratifed cell membrane and its apical tight junctions between surface epithelial cells are considered to be the most prominent barrier for corneal absorption. Topical delivery into the conjunctival cul-de-sac is by far the most common route of ocular drug [2]. Absorption from this site may be corneal or noncorneal. Te corneal absorption represents the major mechanism of absorption for most therapeutic entities. Te cornea is a trilaminate structure consisting of three major difusional barriers, epithelium, stroma, and endothelium [3]. Nonsteroidal anti-infammatory drugs (NSAIDs) are recommended for treatment of various pains, infammatory conditions of eye, osteoarthritis, and rheumatoid arthritis. It acts by blocking cyclooxygenase pathway. NSAIDs have proven to be a safe and efective alternative to corticosteroids in the topical management of ocular infammations [4]. For treating ocular diseases, eye drops may be used but requires frequent instillation of highly concentrated solutions due to rapid precorneal loss from the eye. So, a prolonged action pharmaceutical may be recommended. Aceclofenac eye drop is not available in market so in this we use a novel approach to formulate aceclofenac eye drops to reduce eye infam- mation. Currently these drugs are used topically varying widely in inhibition of intraoperative miosis, management of postoperative infammation, treatment of seasonal allergic conjunctivitis, prevention and treatment of cystoid macu- lar edema, and the control of pain afer photo refractive keratectomy. NSAIDs have also been found to be useful in decreasing bacterial colonization of contact lenses and preventing bacterial adhesion to human corneal epithelial cells [5, 6]. Rabbit cornea has been preferred in the majority of the permeation studies which is now been restricted by most of Animal Ethical Committees across the globe. Keeping this view in mind, three diferent corneas of goat, sheep, and bufalo were selected for this study. Corneas were procured from local butcher’s shop at Banasthali, Newai, Rajasthan. Te current study focuses on diferent factors like pH, nature and amount of preservatives, that infuences rate of permeation (in vitro) of an aceclofenac formulation through diferent freshly excised corneas of goat, sheep, and bufalo. Hindawi Publishing Corporation e Scientific World Journal Volume 2015, Article ID 432376, 7 pages http://dx.doi.org/10.1155/2015/432376