http://informahealthcare.com/mdn ISSN: 1940-1736 (print), 1940-1744 (electronic) Mitochondrial DNA, Early Online: 1–2 ! 2013 Informa UK Ltd. DOI: 10.3109/19401736.2013.825786 MITOGENOME ANNOUNCEMENTS The mitochondrial genome of Diadromus collaris (Hymenoptera: Ichneumonidae) Qian Li 1 , Shu-jun Wei 1,2 , Min Shi 1 , and Xue-Xin Chen 1 1 State Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, Hangzhou, China and 2 Institute of Plant and Environmental Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China Abstract We sequenced the nearly complete mitochondrial genome of the parasitic wasps Diadromus collaris, the fourth sequenced mitogenome in the family Ichneumonidae (Insecta: Hymenoptera). The sequenced segment is 14,621 bp, including 13 protein-coding genes, 19 transfer RNA genes and 2 ribosomal RNA genes. Four tRNAs are rearranged comparing to the ancestral insect mitochondrial gene arrangements, which coincides with the fact that the most rearranged genes are tRNA genes in the Ichneumonidae, and trnI-trnQ-trnM is a hot-spot of gene rearrangement. The lrRNA secondary structure was predicted, containing six domains (I–VI) and 49 helics. Keywords Diadromus collaris, lrRNA, mitochondrial genome, secondary structure History Received 11 July 2013 Accepted 13 July 2013 Published online 18 September 2013 The mitochondrial genome of Diadromus collaris was sequenced (GenBank accession No. JX131613) using the traditional PCR amplification method (Wei et al., 2009). This is the fourth sequenced mitogenome in the family Ichneumonidae (Insecta: Hymenoptera). The sequenced segment is 14,621 bp long. Nineteen tRNAs, 13 protein-coding genes and 2 rRNAs were fully sequenced while the sequences between srRNA and trnQ including A þ T-rich region, trnF, trnM and trnI were not. The failure to sequence the A þ T-rich region may be due to the high A þ T content and the presence of repeats. This phenomenon is very common in Hymenoptera even using 454 sequencing method (Castro & Dowton, 2005; Castro et al., 2006; Mao et al., 2012; Oliveira et al., 2008; Wei et al., 2010). There are 13 overlapping regions between the genes ranging from 1 bp to 9 bp, and 12 non-coding regions ranging from 1 bp to 71 bp (Table 1). Except for A þ T-rich region, there are some long non-coding regions between genes in four sequenced ichneumo- nid mitogenomes, where the longest non-coding region is 71 bp between trnT and ND6 in D. collaris, 1515 bp between cox1 and cox2 in Diadegma semiclausum (Wei et al., 2009), 68 bp between trnW and trnY in Venturia canescens and 61 bp between trnR and trnE in Enicospilus sp. (Dowton et al., 2009). The A þ T content of D. collaris partial mitochondrial genome is 83.97%, A for 41.84% and T for 42.13%. The lengths of the 19 tRNAs range from 63 bp to 83 bp. Among the 19 tRNAs, seven are encoded on the minority strand. The position and gene length of lrRNA and srRNA are conserved. The length of lrRNA is 1371 bp with 87.39% A þ T content. The A þ T content of the partial rrnS is 85.42%. We predicted the lrRNA secondary structure of D. collaris which contains six domains (I–VI) and 49 helics. Correspondence: Xue-Xin Chen, Institute of Insect Sciences, Zhejiang University, C1441 Agro-Sciences Building, 866 Yuhangtang Road, Hangzhou 310058, China. Tel: +86 571 88982868. E-mail: xxchen@ zju.edu.cn Table 1. Annotation of the Diadromus collaris mitochondrial genome. Gene Strand Gene position Length (bp) Anti/start codon Stop codon Intergenic nucleotides trnQ  80–148 69 TTC ND2 þ 162–1151 990 ATA TAA 13 trnW þ 1150–1225 76 TCA 2 trnY  1251–1317 67 GTA 25 trnC  1327–1390 64 GCA 9 cox1 þ 1391–2927 1543 ATG T 7 trnL2 þ 2928–2991 64 TAA 0 cox2 þ 2992–3673 681 ATT T 0 trnK þ 3674–3744 71 CTT 0 trnD þ 3746–3812 67 GTC 1 atp8 þ 3813–3965 152 ATT TAA 0 atp6 þ 3959–4633 674 ATG TAA 7 cox3 þ 4638–5426 788 ATG TAA 4 trnG þ 5426–5491 66 TCC 1 ND3 þ 5492–5845 353 ATA TAA 0 trnA þ 5836–5918 83 TGC 9 trnR þ 5915–5979 65 TCG 3 trnN þ 5979–6045 67 GTT 1 trnS1 þ 6039–6112 74 TCT 6 trnE þ 6114–6177 64 TTC 1 trnP  6220–6289 70 GAA 42 ND5  6289–7965 1676 ATG TAA 1 trnH  7967–8033 67 GTG 1 ND4  8032–9358 1327 ATG T 2 ND4l  9352–9645 294 ATT TAA 7 trnT þ 9647–9709 63 TGT 1 ND6 þ 9781–10,344 563 ATT TAA 71 Cytb þ 10,344–11,477 1133 ATG TAG 1 trnS2 þ 11,476–11,542 67 TGA 1 ND1  11,549–12,463 914 ATA TAA 6 trnL1  12,493–12,559 67 TAG 29 lrRNA  12,560–13,930 1371 0 trnV  13,931–13,997 67 TAC 0 srRNA  13,998–14,621 623 0 þ, majority strand; , minority strand. Mitochondrial DNA Downloaded from informahealthcare.com by Zhejiang University on 09/24/13 For personal use only.