2013 http://informahealthcare.com/mdn ISSN: 1940-1736 (print), 1940-1744 (electronic) Mitochondrial DNA, Early Online: 1–2 ! 2013 Informa UK Ltd. DOI: 10.3109/19401736.2013.803542 MITOGENOME ANNOUNCEMENTS Complete mitochondrial genome of Neochauliodes bowringi (MacLachlan) (Megaloptera: Corydalidae) Qian Li 1 , Shu-jun Wei 1,2 , Min Shi 1 , and Xue-xin Chen 1 1 State Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, Hangzhou, China and 2 Institute of Plant and Environmental Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China Abstract We describe and analyze the complete mitochondrial genome of Neochauliodes bowringi (MacLachlan) (Megaloptera: Corydalidae). The length of the genome is 16,064 bp (GenBank accession No. JQ351950), the longest mitochondrial genome in the order Megaloptera already got, which includes 22 transfer RNA genes, 13 protein-coding genes, two ribosomal RNAs and an A þ T-rich region. The gene arrangement is similar to that of Drosophila yakuba, the presumed ancestral insect mitochondrial gene arrangement. All protein-coding genes start with ATN start codon except for the gene ND4, which uses GTG as start codon. Eight protein-coding genes use TAA as stop codon and others use incomplete stop codons ‘‘T’’ or ‘‘TA’’. The A þ T-rich region is located between rrnS and trnI with a length of 1328 bp. Keywords Megaloptera, mitochondrial genome, Neochauliodes bowringi, Neuroptera History Received 30 April 2013 Accepted 6 May 2013 Published online 16 July 2013 The complete mitochondrial genome of Neochauliodes bowringi (MacLachlan) was determined (GenBank accession No. KC414791) using the traditional PCR amplification method (Wei et al., 2009). Both strands were determined by primer walking. The length of this mitogenome is 16,064 bp, the longest mitochondrial genome in the order Megaloptera already got (Beckenbach & Stewart, 2009; Cameron et al., 2009; Hua et al., 2008; Wang et al., 2012). It contains 37 typical animal mitochondrial genes, including 13 protein-coding, 2 rRNA and 22 tRNA gene, and an A þ T-rich region (Table 1). The gene arrangements are similar to those of D. yakuba, the presumed ancestral insect mitochondrial gene order (Clary et al., 1982). There are 16 overlapping regions between neighboring genes, ranging from 1 bp to 8 bp, and the overlapping nucleotides are totally 53 bp while there are 10 non-coding regions, ranging from 2 bp to 12 bp, and the intergenic spacers are totally 57 bp. The A þ T content of this mitogenome is 76.55%, A for 39.67% and T for 36.88%. The AT-skew and GC-skew is 0.0364 and 0.2768 separately, indicating that more A than T and more C than G are present in the genome. The position and gene length of the tRNA and rRNA genes are conserved. The length of the 22 tRNA genes ranges from 62 bp to 71 bp with typical cloverleaf structures. The length of the lrRNA is 1386 bp with 81.60% of A þ T content. The length of the srRNA is 784 bp with 79.34% of A þ T content. The protein-coding genes are totally 11,153 bp long. The size of each protein-coding gene is similar to that of their orthologs in other insects. The A þ T content of the protein-coding genes is 73.64%. The start codons of all protein-coding genes are ATN, except for nad4, which uses GTG as the start codon. In the presently sequenced insect mitochondrial genomes, GTG start codon of the nad4 gene was previously only found in Atelura Correspondence: Xue-xin Chen, Institute of Insect Sciences, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China. Tel: +86 571 88982868. E-mail: xxchen@zju.edu.cn Table 1. Annotation of the Neochauliodes bowringi mitochondrial genome. Gene Strand Gene position Gene length (bp) Anti/start codon Stop codon Intergenic nucleotides trnI þ 1–63 63 GAT trnQ  129–61 69 TTG 3 trnM þ 142–210 69 CAT 12 ND2 þ 211–1227 1017 ATT TAA 0 trnW þ 1226–1290 65 TCA 2 trnC  1344–1283 62 GCA 8 trnY  1409–1345 65 GTA 0 cox1 þ 1402–2942 1541 ATT TA 8 trnL2 þ 2943–3006 64 TAA 0 cox2 þ 3012–3692 1017 ATG TAA 5 trnK þ 3701–3771 71 CTT 8 trnD þ 3771–3836 66 GTC 1 atp8 þ 3837–3995 159 ATC TAA 0 atp6 þ 3989–4666 678 ATG TAA 7 cox3 þ 4666–5452 787 ATG T 1 trnG þ 5457–5519 63 TCC 4 ND3 þ 5520–5871 352 ATT T 0 trnA þ 5872–5935 64 TGC 0 trnR þ 5947–6009 63 TCG 12 trnN þ 6009–6075 67 GTT 1 trnS1 þ 6075–6143 69 GCT 1 trnE þ 6143–6208 66 TTC 1 trnF  6271–6207 65 GAA 2 ND5  6277–8000 1724 ATT T 5 trnH  8064–7998 67 GTG 3 ND4  8065–9400 1335 GTG T 0 ND4l  9394–9684 291 ATG TAA 7 trnT þ 9687–9752 66 TGT 2 trnP  9818–9753 66 TGG 0 ND6 þ 9824–10,333 510 ATT TAA 5 Cytb þ 10,333–11,469 1137 ATG TAA 1 trnS2 þ 11,468–11,534 67 TGA 2 ND1  11,547–12,494 948 ATA TAA 2 trnL1  12,556–12,493 64 TAG 5 lrRNA  12,494–13,879 1386 0 trnV  13,950–13,880 71 TAC 0 srRNA  13,953–14,736 783 2 CR 14,737–16,064 1328 0 þ, majority strand; , minority strand; CR, A þ T rich region. 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