1 Volume: 2017; Issue: 05 Archives on Veterinary Science and Technology Case Report Mohammed A, et al. Arch Vet Sci Technol, 2017: AVST-121. A Comparison of Eggs Per Gram (Epg) from Wet Faecal Smears Stan- dard Method, with Benchtop Flotation with Centrifugal Techniques in Determining the Number of GIN Eggs in Calf Faeces-A Case Study Aphzal Mohammed * , Edward Sampson, Puran Bridgemohan, Angelisah Khan The University of Trinidad and Tobago, ECIAF, Caroni North Bank Road, Centeno Arima, West Indies * Corresponding author: Aphzal Mohammed, Assistant Professor, The University of Trinidad and Tobago, ECIAF, Caroni North Bank Road, Centeno Arima,West Indies; Tel: +868-642 8888; E-mail: aphzal.mohammed@utt.edu.tt Citation: Mohammed A, Sampson E, Bridgemohan P, Khan A (2017) A Comparison of Eggs Per Gram (Epg) from Wet Faecal Smears Standard Method, with Benchtop Flotation with Centrifugal Techniques in Determining the Number of GIN Eggs in Calf Faeces-A Case Study. Arch Vet Sci Technol, 2017: AVST-121. DOI:10.29011/AVST-121/100021 Received Date: 11 June, 2017; Accepted Date: 17 June, 2017; Published Date: 25 June, 2017 Abstract Aim: Although several techniques exist for quantifying GastroIntestinal Nematode eggs (GIN), the method producing the highest egg counts would result in better targeting of parasitic control in livestock. Materials and Techniques: An experiment was therefore, conducted to determine the best technique in quantifying Gas- trointestinal (GIN) helminths eggs in calf faeces by comparing the wet faecal smear, faecal foatation and centrifugation foatation techniques offaeces of a moderately parasitized calfnfested withan initial egg per gram (epg) of nine hundred and ffty (950). Seven (7) techniques were used and observations recorded in triplicate by separate observers.The solutions used for the quantifcation of were sodium chloride (NaCl), zinc sulphate (ZnSO4) and sucrose. The average eggs count from six techniques were tested and fndings using descriptive statistics and an ANOVA due to technique are reported. Results: Descriptive statistics showed that centrifugation foatation techniques showed the highest quantifcation of GIN eggs. Egg counts by centrifugation were highest using foatation solutions sodium chloride followed by sucrose then zinc sulphate. These fndings corresponded with high the signifcant difference (P<0.0001) found among techniques used. Conclusion: Therefore, we recommend that centrifugation techniques be used for quantifying GIN eggs in targeting follow up anthelmintic treatment in livestock affected by gastrointestinal parasites. Keywords: GIN eggs, Solus, Eggs per gram, Faeces, Centrifu- gation, Floatation Parasitic infections of livestock pose a serious problem for farmers, and a major obstacle for the development and expansion of the meat industry into a sustainable and proftable market [1]. The direct losses caused by these parasites are usually attributed to acute illness and death, premature slaughter and rejection of some parts at meat inspection. Indirect losses include the diminution of productive potential such as decreased growth rate, weight loss in young growing animals, inconsistent reproductive cycling, late maturity of slaughter stock and poor milk production [2,3]. These infections can be either clinical or sub clinical, the latter being the most prevalent and of great economic signifcance. The economic losses of parasitic infections vary from loss of condition of animals to loss of production. These GIT parasitic worms belong to the Phyla Platyhelmint- hes, Nemathelminthes or Nematoda, Acanthocephala and Annelida [4].The Phylum Platyhelminthes contains three classes of worms namely; Turbellaria, Trematoda and Cestoda. All three classes are typically soft-bodied, fattened dorso-ventrally and hermaphro- ditic. Of major importance however, to grazing livestock are the Nematodes commonly called roundworms, from their appearance in cross-section. The nematode eggs however, differ greatly in size and shape, and the shell is of variable thickness [5,6]. Note that, Calves under one year of age in tropical environments are more susceptible to nematodes than older animals [7]. DOI:10.29011/AVST-121/100021