Warsinah & Baroroh (2019) Bandotan as anti-bacterial December 2019 Vol. 11 ©Annals of Tropical Medicine & Public Health S317 ISOLATION OF COMPOUNDS AGENTS ANTI BACTERIA FROM BANDOTAN (Ageratum conyzoides L ) PLANTS USING BIOASSAY GUIDED FRACTIONATION METHOD Warsinah Warsinah 1)* , Hanif Nasiatul Baroroh 1) 1) Department of Pharmacy, Faculty of Health Sciences, Jenderal Soedirman University *Corresponding author: Warsinah Warsinah, Department of Pharmacy, Faculty of Health Sciences, Jenderal Soedirman University. Jl.dr. Soeparno, Karangwangkal, Purwokerto, Indonesia. Email: warsinahapt@gmail.com. ABSTRACT Objectives: Bandotan (Ageratum conyzoides L) herbaceous plants have been empirically used for wound medicine and digestive disorders. The treatment is related to its pharmacological activity as an antibacterial. the purpose of this study was to isolate responsible compounds as antibacterial Methods: The research phase is extraction using maceration method, fractionation by liquid vacuum column chromatography followed by TLC, the same profile of TLC combined and then tested for its activity against Esceresia coli and Staphylococus aureus. Results: The results of the antibacterial activity test showed that the inhibition of ethyl acetate extract was 14 mm, the combined fraction 4 was 16 mm and subfraction gab 4 was 14.5 mm at S aureus, whereas in E coli extract the ethylacetate extract was 11 mm, the combined fraction 4 was 13 mm and sub FG4b of 10 mm. The Identification with using UV-Vis, IR and GC-MS spectrophotometers showed the presence of coumarin compounds, 2 kromen compounds (precocene II and 7-methoxy-2,2-dimethyl-6-vinyl-2H-kromen), 2 palmitic acid derivative compounds ( hexadecanoic acid and 9,12-octadecanoic acid), triterpenes (neopitadiene) and methyl-5,11,14,17-eikosatetraenoic compounds Conclusions: The minimum inhibition of combined 4b subfraction against S. aureus is 25 mg / ml and 50 mg / ml for E. coli. Identification compounds of bandotan herbs was contain compounds called coumarin, kromen, fatty acids and 5,11,14,17-methyl eicosatetraenoate Keywords: Ageratum conyzoides, isolation, Bioassay guided fractionation How to cite this article: Warsinah W, Baroroh HN (2019): Isolation of compounds agents antibacterial from Bandotan (Ageratum conyzoides L) plants using bioassay guided fractionation method, Ann Trop & Public Health; 22(11): S317. DOIhttp://doi.org/10.36295/ASRO.2019.221111 INTRODUCTION Empirically medicinal plants have been used by one third of the world's population. One of these plants is bandotan (Ageratum conyzoides), including familli Asteraceae. This plant is a wild plant and as a weed. Bandotan contains compounds at alkaloid, tannins, flavonoids, steroids, terpenoids, coumarins and essential oils, with properties as medicine for wounds, hives, flu, fever, diarrhea, intestinal inflammation, and rheumatism (8). Methanol, ethanol and water extract from bandotan herbs have antibacterial activity (10, 11), until now, the search for antibacterial active compounds from natural materials is still being carried out considering that synthetic drugs such as existing antibiotics can cause problems with the emergence of resistance and not all antibiotics can be given to children (6). Therefore the search for active ingredients based on natural ingredients is unlimited, including the search for new compounds that have pharmacological properties such as