El-Hayah Vol. 6, No.2 (2017) 50-56 JURNAL BIOLOGI e e l l – – H H a a y y a a h h Journal Homepage: http://ejournal.uin-malang.ac.id/index.php/bio/index e-ISSN: 2460-7207, p-ISSN: 2086-0064 Protein Expression of Soybean (Glycine Max L. Merr) Varieties In Drought Stress Evika Sandi Savitri 1 , Estri Laras Arumingtyas 2 1 Department of Biology, Faculty of Science & Technology, UIN Maulana Malik Ibrahim Malang, 65145, 1Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang, 65145 * Corresponding author Email : evikasandi@yahoo.com DOI: 10.18860/elha.v6i2.4822 A r t i c l e I n f o Article history: Received 08 December 2016 Received in revised form 23 August 2017 Accepted 28 December 2017 Keywords: drought, protein, two dimensional gel electrophoresis A b s t r a c t Drought is one of the most severe limitations on the productivity of soybean. There are many genes and proteins involved in drought stress tolerance. Identification of proteins which could be used as the base for the development of molecular study is very important to understand drought tolerance thoroughly. The objective of the research was to investigate protein expression of soybean to drought stress. Changes in protein expression were analyzed using SDS PAGE and two dimensional gel electrophoresis. Image analysis of 2D protein was performed by using the PDQuest 8.0 software program (Bio-Rad). Tolerant variety, Dering-1, was subjected to drought stress using limitation of watering, while Detam-1, a sensitive variety, was used as comparator. The result showed that protein concentration have decreased in drought condition from 3,22 mg/ml to 0,77 mg/ml. The new protein band with the 24,95 kDa have been found in drought condition. This protein is osmotin like protein with the accession number NP915414 which may play a role in the mechanism of drought resistance. The identification of the protein based on sequence amino acid literature review. 1. INTRODUCTION Wetland, characterized by two dry season (I and II), is commonly used as the main agro- ecosystem soybean production in Indonesia. As a result of this condition, soybean cultivation often have the risk of crop failure due to the drought. Global warming causes the increase in the intensity of extreme drought and the risk of such failures. Recently, soybean varieties which are specifically released for the purpose of drought tolerant are not available yet. Varieties Dering-1 (strain DV / 2984-330) is a soybean variety that is assembled to solve the problem. The research on Dering-1 has been performed over six years to solve the risk of