Whole-Genome Sequence of Acinetobacter baumannii Strain NUBRI-A, Isolated from a Hospitalized Patient in Khartoum, Sudan Sofia B. Mohamed, a Mohamed Hassan, a Abdalla Munir, a Sumaya Kambal, a Nusiba I. Abdalla, a Ahmed Hamad, a Sara Mohammed, a Fatima Ahmed, a Omnia Hamid, a Arshad Ismail, b Mushal Allam b a Bioinformatics and Biostatistics Department, National University Biomedical Research Institute, National University, Khartoum, Sudan b Sequencing Core Facility, National Institute for Communicable Diseases, National Health Laboratory Service, Johannesburg, South Africa ABSTRACT Acinetobacter baumannii has emerged as an important pathogen lead- ing to multiple nosocomial outbreaks. Here, we describe the genomic sequence of a multidrug-resistant Acinetobacter baumannii sequence type 164 (ST164) isolate from a hospital patient in Sudan. To our knowledge, this is the first reported draft ge- nome of an A. baumannii strain isolated from Sudan. A cinetobacter baumannii is an aerobic Gram-negative bacillus frequently linked to health care-associated infections. A. baumannii causes a wide spectrum of infec- tions that includes pneumonia, bacteremia, meningitis, and urinary tract infection (1, 2). Its ability to persist for extended periods, in combination with increasing antibiotic resistance, makes it a frequent cause of nosocomial outbreaks. Here, we report the draft genome sequence of an A. baumannii strain (NUBRI-A) isolated from a patient admitted to a private hospital in Khartoum, Sudan. A sputum sample was collected from a 35-year-old male admitted to the Royal Care International Hospital in Khartoum, Sudan, with a pneumonia infection. The specimen was directly inoculated on MacConkey agar and then incubated overnight under aerobic conditions at 37°C. The colony was identified using Gram staining and bio- chemical tests that included oxidase, catalase, Kligler’s iron agar, sulfide indole motility, citrate agar, and urea tests. The analytical profile index was used to confirm the species (3). Genomic DNA was extracted using the QIAamp DNA minikit (Qiagen, Germany), and paired-end libraries were prepared using the Nextera DNA Flex library kit, followed by 2  300-bp sequencing on a MiSeq platform (Illumina, Inc., USA). The sequenced reads were trimmed using Sickle version 1.33 (with the parameters -q 20 -l 75) (https://github .com/najoshi/sickle) and de novo assembled using SPAdes version 3.11 (with the parameters -careful and -cov-cutoff auto) (4). All resultant contiguous sequences were then submitted to the NCBI Prokaryotic Genome Annotation Pipeline (5). Multilocus sequence typing (MLST) was determined using mlst (https://github.com/tseemann/ mlst). This study was approved by the Research Ethics Committee at the National University in Sudan. A total of 1,777,192 paired-end reads were obtained from the whole-genome sequencing of NUBRI-A. Quality-controlled reads (1,768,578 reads; average length, 211 bp) with a Phred score of 20 were assembled. The assembled genome was composed of 33 contigs (all were longer than 200 bp), and the longest contig size was 1,777,326 bp, covering 3,743,802 bp. The GC content and N 50 value were 38.9% and 390,599 bp, respectively. The genome assembly metric was calculated with QUAST version 5.0.2 using default parameters. The total number of genes is 3,576; of those, 3,444 are protein-coding genes, 70 are RNA genes, and 62 are pseudogenes. MLST (Oxford scheme) on this strain revealed that it belongs to sequence type 164 (ST164). Citation Mohamed SB, Hassan M, Munir A, Kambal S, Abdalla NI, Hamad A, Mohammed S, Ahmed F, Hamid O, Ismail A, Allam M. 2019. Whole-genome sequence of Acinetobacter baumannii strain NUBRI-A, isolated from a hospitalized patient in Khartoum, Sudan. Microbiol Resour Announc 8:e00542-19. https://doi.org/10.1128/MRA.00542-19. Editor Irene L. G. Newton, Indiana University, Bloomington Copyright © 2019 Mohamed et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. Address correspondence to Sofia B. Mohamed, sofiabashir2002@gmail.com. Received 9 May 2019 Accepted 23 July 2019 Published 8 August 2019 GENOME SEQUENCES crossm Volume 8 Issue 32 e00542-19 mra.asm.org 1