Temporal Expression Patern of Peptdes in the Regeneratng Caudal Fin of Teleost Fish Poecilia latpinna With Special Emphasis on krt15 and myl-1 Hiral Murawala, Isha Ranadive, Sonam Patel and Suresh Balakrishnan * Department of Zoology, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara, Gujarat, India Corresponding author: Suresh Balakrishnan, Department of Zoology, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara, Gujarat, India, Tel: 2656592311; E-mail: suved9@hotmail.com Received Date: July 05, 2017; Accepted Date: July 25, 2017; Published Date: August 15, 2017 Copyright: © 2017 Balakrishnan S, et al. This is an open-access artcle distributed under the terms of the Creatve Commons Atributon License, which permits unrestricted use, distributon, and reproducton in any medium, provided the original author and source are credited. Citaton: Murawala H, Ranadive I, Patel S, Balakrishnan S (2017) Temporal Expression Patern of Peptdes in the Regeneratng Caudal Fin of Teleost Fish Poecilia latpinna With Special Emphasis on krt15 and myl-1. Eur Exp Biol. Vol. 7 No. 4:21. Abstract Amongst several vertebrates, caudal fn of teleost fsh has emerged as an excellent model to understand the mechanism of regeneraton. By now it is well perceived that proper coordinaton of diferent regulatory signals are needed to facilitate the progression of successive stages of regeneraton. Therefore, in the current study protein expression profle for caudal fn regeneraton in Poecilia latpnna was brought into the focus. The patern of protein turnover and variaton in the expression level were observed across the stages of regeneraton. Data from two- dimensional gel electrophoresis revealed the diferental expression of proteins for wound epithelium, blastema and diferentaton stages when compared to restng stage. Based on the computatonal analysis, of the peptde expression profle and subsequent sequencing, two peptdes namely Keratn type I cytoskeletal 15 and Myosin light chain-1 were studied further at transcript level. Keratn-15 was found to be up regulated at wound epithelium stage while its expression waned signifcantly in the blastema and diferentaton stages of regeneraton indicatng its role in the formaton of a functonal wound epithelium. Western blot analysis of Keratn-15 also concurred with transcript levels of krt15. However, myl-1 transcript levels was observed to be dispensable for inital phase of regeneraton whereas its elevated level in diferentaton stage at both mRNA and protein level marks its noteworthy role in achieving the structural integrity of a regeneratng caudal fn. This is the frst observaton of the involvement of Keratn-15 and Myosin light chain-1 in the regulaton of regeneratve process in Poecilia latpinna. Keywords: Two-dimensional gel electrophoresis; Caudal fn regeneraton; Teleost fsh; P. latpinna; Krt15 and myl-1. Introducton Epimorphic regeneraton involves the de-diferentaton of existng tssue and recruitment of proliferatve cells to form the new structure to restore the lost appendage. Such type of appendage regeneraton is observed in a number of vertebrates including lizard tail, salamander limb and tail [1-4], and zebrafsh caudal fn [5-7]. Amongst the models of vertebrate appendage regeneraton, teleost caudal fn has emerged as an extensively used model to study epimorphic regeneraton. Most of the caudal fn regeneraton studies have been carried out in zebrafsh due to its accessibility, its fast and robust regeneraton and its simple architecture [8,9]. However, sailfn molly, Poecilia latpnna (Lesueur, 1821) have been served as an alternatve to zebrafsh, being more adaptve to the oriental laboratory conditons as well as certain experimental advantages owing to its relatvely large size. Following amputaton of caudal fn in zebrafsh, an epithelial layer covers the wound which gets thickened later on. Following this, mesenchymal cells migrate near the amputaton plane beneath the newly formed wound epithelium, accumulate and proliferate to form the blastema and fnally diferentate to replace the lost structures [5,9,10-14]. Wound healing or wound epithelium formaton is achieved within 12-24 hours afer amputaton and blastema formaton is achieved in 48-60 hours post amputaton. Finally, the blastemal cells diferentate and restore structures including blood vessels, bony rays, and connectve tssue by 15-20 days post amputaton [6,9,12,15]. This tme course of caudal fn regeneraton in P. latpinna difers to that of zebrafsh which has been reported in this study for the frst tme. The aforementoned stages of epimorphic regeneraton are governed by certain cellular events which are ultmately regulated at molecular level. For each process to occur in a spato-temporal manner, a large scale of protein turnover takes place. During protein turnover, several peptdes need to be synthesized newly as per the need of the tssue and many to be downregulated. There are reports on proteomic profle in regeneraton models to unravel the conserved and diverse mechanisms amongst them [16-19]. It includes the regeneraton in axololtl [20,21], Xenopus laevis froglet limb-bud [22,23] and zebrafsh caudal fn regeneraton [24]. Herein, in P. latpinna, two- dimensional gel electrophoresis was used to fnd out the overall expression patern of peptdes across the stages of epimorphic regeneraton. This was followed by peptde sequencing of Research Article iMedPub Journals http://www.imedpub.com/ DOI: 10.21767/2248-9215.100021 Open Access ISSN 2248-9215 Vol.7 No.4:21 2017 © Under License of Creative Commons Attribution 3.0 License | This article is available from: http://www.imedpub.com/european-journal-of-experimental- biology/ 1