TETRAHEDRON LETTERS Tetrahedron Letters 44 (2003) 8383–8386 Pergamon Monoalkylation of dihydroxycoumarins via Mitsunobu dehydroalkylation under high intensity ultrasound. The synthesis of ferujol Giancarlo Cravotto, a, * Stefano Chimichi, b, * Bruna Robaldo a and Marco Boccalini b a Dipartimento di Scienza e Tecnologia del Farmaco, Universita ` di Torino, via Giuria 9, 10125 Torino, Italy b Dipartimento di Chimica Organica U. Schiff, Universita ` di Firenze, via della Lastruccia 13, I -50019 Sesto F.no, Firenze, Italy Received 17 July 2003; revised 8 September 2003; accepted 16 September 2003 Abstract—Monoalkylation of natural dihydroxycoumarins was carried out by Mitsunobu dehydroalkylation under sonochemical conditions. Aesculetin (6,7-dihydroxycoumarin) was selectively alkylated in good yield with prenyl alcohols at position 7, as clearly shown by NOESY experiments; though less selectively, position 7 was also the most reactive in daphnetin (7,8-dihydroxy- coumarin). The synthesis of the phytoestrogen ferujol is also reported for the first time. © 2003 Elsevier Ltd. All rights reserved. 1. Introduction The Mitsunobu reaction 1 has been exploited in the chemistry of a wide range of naturally occurring com- pounds like carbohydrates, 2 peptides, 3 terpenes 4 and others with formation of ethers, esters and new CC bonds. 5 The nucleophilic substitution of an alcohol group mediated by the triaryl- or trialkyl-phosphine/ dialkyl azodicarboxylate redox system is widely used to prepare biologically active compounds with interesting applications in solid phase synthesis 6 and combinatorial chemistry. 7 The regio- and stereoselectivity of the reac- tion have been thoroughly reviewed. 5 Although its reac- tion mechanism with saturated alcohols is well known, uncertainty lingers on the factors that govern its course when allyl or prenyl alcohols are employed. Although allylic alcohols react according to the normal S N 2 mechanism, some instances are found in the literature of an S N 2mechanism. 5 The reaction normally proceeds with inversion of configuration at the carbon atom bearing the hydroxy group, yet complete retention of configuration has been observed in the lactonization of hindered alcohols. 8 As regards regioselectivity, Ko 9 found that with syn -2,3-dihydroxy esters only the - hydroxy group reacted. On the other hand, with 1,3- dicarbonyl compounds, the reaction yields mixtures of C - and O -alkylation products because the enolate charge is delocalized. 10 It is well known that 4-hydroxy- coumarin usually gives O -alkylation products. 11 In a recent paper 12 we compared the outcomes of the reac- tion under conventional and sonochemical conditions, showing that high intensity ultrasound influences only slightly the regioselectivity, while markedly increasing reaction rates and yields. Our preliminary data, 13 fur- ther developed by Appendino, 14 indicate that the Mit- sunobu reaction affords an easy way to perform a differential esterification of alcoholic and phenolic hydroxy groups. We have now found that the Mitsunobu reaction allows a differential etherification of the phenolic groups in dihydroxycoumarins. Although phenol etherification has been widely exploited, 15 to the best of our knowl- edge, this result has never been published. Scheme 1. Reagents and conditions :(i ) ROH, PPh 3 , DIAD, THF, )))) 45 min or stirring at 20°C for 5–8 h; (ii ) Ac 2 O, pyridine. Keywords : dihydroxycoumarins monoalkylation; high intensity ultra- sound; Mitsunobu dehydroalkylation; ferujol synthesis. * Corresponding authors. Tel.: +39 (0)55 457 3537; fax: +39 (0)55 457 3568; e-mail: stefano.chimichi@unifi.it 0040-4039/$ - see front matter © 2003 Elsevier Ltd. All rights reserved. doi:10.1016/j.tetlet.2003.09.114