ORIENTAL JOURNAL OF CHEMISTRY
www.orientjchem.org
An International Open Access, Peer Reviewed Research Journal
ISSN: 0970-020 X
CODEN: OJCHEG
2019, Vol. 35, No.(3):
Pg. 1182-1186
This is an Open Access article licensed under a Creative Commons license: Attribution 4.0 International (CC- BY).
Published by Oriental Scientific Publishing Company © 2018
Electrochemical Detection of Dissolved Sulphur Dioxide Using
Bovine Serum Albumin - Theoretical and Experimental Approach
SHRUTHI GIRIDHARAN
1
, JEMINI JOSE
2
and SREEJA. P. B
1
*
1,2
Department of Chemistry, CHRIST (Deemed to be University), Hosur Road, Bangalore 560029, India.
*Corresponding author E-mail: sreeja.pb@christuniversity.in
http://dx.doi.org/10.13005/ojc/350338
(Received: May 10, 2019; Accepted: June 11, 2019)
ABSTRACT
In this research work, we discuss the fabrication of an electrochemical sensor that can
successfully detect 10
-3
M concentration of sulphur dioxide. Electrochemical analyses were carried
out using glassy carbon electrode modified with the protein – bovine serum albumin. The studies
were carried out experimentally and theoretically. The theoretical studies were by molecular docking
of bovine serum albumin with sulphur dioxide and experimentally, cyclic voltammetry was performed
for different scan rates and concentrations of sulphur dioxide. The results obtained prove that bovine
serum albumin has appreciable interaction with sulphur dioxide and hence can be used as a basis
for the detection of the same.
Keywords: Sulphur dioxide, Electrochemical detection, Bovine serum albumin,
Fabrication of electrodes.
INTRODUCTION
Sulphur dioxide is a compound whose
natural state of existence is in the gaseous form.
This particular compound is a pollutant that poses
a major threat to the environment
1
. Sulphur dioxide
gas is a common gas in vehicular emissions. It is
also generated in-situ in those food products which
have food adulterants comprising sulphite salts, as
they on oxidation produce sulphur dioxide. Sulphur
dioxide being a polar compound exhibits polarity due
to the varying electronegativity in the compound.
This property of the compound is employed in
development of electrochemical sensor that is in-built
with a modified electrode saturated with a compound
that has high affinity and exhibits molecular
interaction with the sulphur dioxide molecule. The
compound that was used to modify the electrode
was bovine serum albumin.
The advent of the work on sulphur dioxide
began with the study of the effects that sulphur
dioxide had on various aspects of plant life processes
such as photosynthesis, respiration, and chlorophyll
content
1
. The kinetics of the homogeneous gaseous
oxidation of sulphur dioxide was studied by Sadek
et al., which was performed in the presence of
catalysts
2
. A new surface acoustic wave gas sensor
was also constructed to detect the presence of
sulphur dioxide and this was based on a polyaniline/
In
2
O
3
nanofibre composite
3
.