S1 Supporting information for Development of a Near-infrared Fluorescent Probe for Imaging of Endogenous Cu + in Live Cells Xiaowei Cao, Weiying Lin,* and Wei Wan State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082, P. R. China E-mail: weiyinglin@hnu.edu.cn Table of contents Page 1. Material and instrument……………………………......……….............S2 2. Synthesis……………….……..……………...………………..…..........S2 3. Cell culture and fluorescence imaging…...………………..…............... S2 4. Preparation of the test solution………….. ………………..…............... S3 5. Figure S1…………………..….…………………………………………S3 Figure S2-3.………………..………………………………………..……S4 Figure S4…………………..……………………………………………..S5 Figure S5…………………..……………………………………………..S6 6. References.……………………..………………………………..............S6 7. NMR spectra………………..……………………………….....................S7 Electronic Supplementary Material (ESI) for Chemical Communications This journal is © The Royal Society of Chemistry 2012