International Journal of Organic Chemistry, 2015, 5, 37-47 Published Online March 2015 in SciRes. http://www.scirp.org/journal/ijoc http://dx.doi.org/10.4236/ijoc.2015.51005 How to cite this paper: Mousa, B.A., Bayoumi, A.H., Korraa, M.M., Assy, M.G. and El-Kalyoubi, S.A. (2015) A Novel One-Pot and Efficient Procedure for Synthesis of New Fused Uracil Derivatives for DNA Binding. International Journal of Organic Chemistry, 5, 37-47. http://dx.doi.org/10.4236/ijoc.2015.51005 A Novel One-Pot and Efficient Procedure for Synthesis of New Fused Uracil Derivatives for DNA Binding Bothaina A. Mousa 1 , Ashraf H. Bayoumi 2* , Makarem M. Korraa 1 , Mohamed G. Assy 3 , Samar A. El-Kalyoubi 4 1 Department of Organic Chemistry, Faculty of Pharmacy, Cairo University, Giza, Egypt 2 Department of Organic Chemistry, Faculty of Pharmacy (Boys), Al-Azhar University, Cairo, Egypt 3 Department of Organic Chemistry, Faculty of Science, Zagazig University, Zagazig, Egypt 4 Department of Organic Chemistry, Faculty of Pharmacy (Girls), Al-Azhar University, Cairo, Egypt Email: s.elkalyoubi@hotmail.com Received 22 December 2014; accepted 25 March 2015, published 27 March 2015 Copyright © 2015 by authors and Scientific Research Publishing Inc. This work is licensed under the Creative Commons Attribution International License (CC BY). http://creativecommons.org/licenses/by/4.0/ Abstract Hydrazinolysis of 6-chloro-1-methyluracil followed by condensation of the product with different aromatic aldehyde gives the respective hydrazones which undergoes oxidative cyclization using thionyl chloride to obtain pyrazolo[3,4-d]pyrimidines in good yields. On the other hand, nitrosa- tion of 6-aminouracils followed by the reaction with different arylidineanilines gives new xan- thine derivatives. Finally, indenopyrrolopyrimidine and indenopteridine are obtained in good yields via the reaction of 6-aminouracils and 5,6-diaminouracil with ninhydrin respectively. The newly synthesized compounds show binding, chelation and fragmentation of the nucleic acid DNA. Keywords 6-Chloro-1-methyluracil, Pyrazolo[3,4-d]pyrimidines, 6-Aminouracils, Xanthine, Indenopyrrolopyrimidine and Indenopteridine 1. Introduction The importance of fused pyrimidines, common source for the development of new potential therapeutic agents [1] [2], is well known. Fused pyrimidines continue to attract considerable attention because of their great practical usefulness, pri- marily due to very wide spectrum of biological activities. This is evident especially from publications of regular