_____________________________________________________________________________________________________ *Corresponding author: E-mail: renner4nrior@gmail.com; Asian Journal of Biology 6(4): 1-8, 2018; Article no.AJOB.43896 ISSN: 2456-7124 Ecotoxicological Assessment of Nigeria Locally Refined Diesel and Kerosene on Aspergillus niger a Key Fungal Pollution Biomarker Renner Renner Nrior 1* , Nedie Patience Akani 1 and Alfred Wilcox 1 1 Departmentof Microbiology, Faculty of Science, Rivers State University, Port Harcourt, Nigeria. Authors’ contributions This work was carried out in collaboration between all authors. Author RRN designed the study, author NPA performed the statistical analysis, wrote the protocol, author AW wrote the first draft of the manuscript. Authors NPA, RRN and AW managed the analyses of the study. Author AW managed the literature searches. All authors read and approved the final manuscript. Article Information DOI: 10.9734/AJOB/2018/43896 Editor(s): (1) Dr. Xing Li, Division of Biomedical Statistics and Informatics, Department of Health Sciences Research, Mayo Clinic College of Medicine, USA. Reviewers: (1) Fábio Henrique Portella Corrêa de Oliveira, Universidade Federal Rural de Pernambuco, Brazil. (2) Mostafa M. Abo Elsoud, Egypt. (3) Anonymous, Nnamdi Azikiwe University, Nigeria. Complete Peer review History: http://www.sciencedomain.org/review-history/26524 Received 5 th July 2018 Accepted 26 th September 2018 Published 5 th October 2018 ABSTRACT Aim: To evaluate the effect of locally refined diesel and kerosene on Aspergillus niger a key fungal pollution biomarker in three aquatic bodies (marine, brackish and freshwater). Study Design: The study employs experimental examination and statistical analysis of the data and interpretation. Place of Study: Fresh water, brackish water, and marine water samples were collected in sterile bottles from Ugama Ekede Stream, Ugama Ekede River and at the foot of the Atlantic ocean in Udun Ama all in Andoni Local Government Area Rivers State, using sterile sampling bottles. These samples were transported to the microbiological laboratory with ice pack within 24 hours for both isolation of test organisms and toxicity. Methodology: Standard microbiological techniques were used; toxicity testing procedures were carried out by preparing locally refined diesel and kerosene at concentrations of 0%, 5%, 10%, 25%, and 50%, tested for durations of 0 h, 24 h, 48 h, 72 h, 96 h. The cultures were incubated at 35°C for 48 hours. LC 50 was determined. Original Research Article