Downloaded from www.microbiologyresearch.org by IP: 54.70.40.11 On: Sun, 02 Dec 2018 20:44:58 Thermosulfurimonas dismutans gen. nov., sp. nov., an extremely thermophilic sulfur-disproportionating bacterium from a deep-sea hydrothermal vent A. I. Slobodkin, 1 A.-L. Reysenbach, 2 G. B. Slobodkina, 1 R. V. Baslerov, 3 N. A. Kostrikina, 1 I. D. Wagner 2 and E. A. Bonch-Osmolovskaya 1 Correspondence Alexander Slobodkin aslobodkin@hotmail.com 1 Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospect 60-letiya Oktyabrya 7/2, 117312 Moscow, Russia 2 Department of Biology and Center for Life in Extreme Environments, Portland State University, PO Box 751, Portland, OR 97207-0751, USA 3 Bioengineering Center, Russian Academy of Sciences, Prospect 60-letiya Oktyabrya 7/1, 117312 Moscow, Russia An extremely thermophilic, anaerobic, chemolithoautotrophic bacterium (strain S95 T ) was isolated from a deep-sea hydrothermal vent chimney located on the Eastern Lau Spreading Center, Pacific Ocean, at a depth of 1910 m. Cells of strain S95 T were oval to short Gram-negative rods, 0.5– 0.6 mm in diameter and 1.0–1.5 mm in length, growing singly or in pairs. Cells were motile with a single polar flagellum. The temperature range for growth was 50–92 6C, with an optimum at 74 6C. The pH range for growth was 5.5–8.0, with an optimum at pH 7.0. Growth of strain S95 T was observed at NaCl concentrations ranging from 1.5 to 3.5 % (w/v). Strain S95 T grew anaerobically with elemental sulfur as an energy source and bicarbonate/CO 2 as a carbon source. Elemental sulfur was disproportionated to sulfide and sulfate. Growth was enhanced in the presence of poorly crystalline iron(III) oxide (ferrihydrite) as a sulfide-scavenging agent. Strain S95 T was also able to grow by disproportionation of thiosulfate and sulfite. Sulfate was not used as an electron acceptor. Analysis of the 16S rRNA gene sequence revealed that the isolate belongs to the phylum Thermodesulfobacteria. On the basis of its physiological properties and results of phylogenetic analyses, it is proposed that the isolate represents the sole species of a new genus, Thermosulfurimonas dismutans gen. nov., sp. nov.; S95 T (5DSM 24515 T 5VKM B-2683 T ) is the type strain of the type species. This is the first description of a thermophilic micro- organism that disproportionates elemental sulfur. Biogeochemical cycling of sulfur in aquatic environments includes the activities of different aerobic and anaerobic prokaryotes. Bacteria that disproportionate sulfur com- pounds such as thiosulfate or elemental sulfur (Bak & Cypionka, 1987; Thamdrup et al., 1993) are a unique group of sulfur cycle micro-organisms. Sulfur isotope data from early Archaean rocks and the presence of microfossils in 3.4-billion-year-old geological formations suggest that sulfur disproportionation could be one of the earliest modes of microbial metabolism (Philippot et al., 2007; Wacey et al., 2011). Inorganic sulfur fermentation has been reported for members of the mesophilic genera Desulfovibrio, Desulfobulbus, Desulfocapsa, Desulfonatronum, Desulfonatronospira and Desulfonatronovibrio in the Delta- proteobacteria (Bak & Pfennig, 1987; Lovley & Phillips, 1994; Janssen et al., 1996; Pikuta et al., 2003; Sorokin et al., 2008, 2011). Among thermophiles, Desulfotomaculum thermo- benzoicum is the only micro-organism that has been reported to be capable of growth by thiosulfate dispropor- tionation (Jackson & McInerney, 2000). Prior to this report, no thermophiles were known to disproportionate elemental sulfur. S 0 is abundant in thermal ecosystems, including deep-sea hydrothermal vents, where it forms when hydrogen sulfide-rich hydrothermal fluid mixes with cold oxygenated seawater (Jannasch, 1984). In these extreme environments, microbial sulfur disproportionation may represent a pre- viously unrecognized process of primary organic matter production. Strain S95 T was isolated from a sample of the actively venting hydrothermal sulfidic chimney-like deposit. The Abbreviation: CFA, cellular fatty acid. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain S95 T is JF346116. A supplementary table and a supplementary figure are available with the online version of this paper. International Journal of Systematic and Evolutionary Microbiology (2012), 62, 2565–2571 DOI 10.1099/ijs.0.034397-0 034397 G 2012 IUMS Printed in Great Britain 2565