_____________________________________________________________________________________________________ *Corresponding author: E-mail: bashirfomda@gmail.com; Journal of Advances in Medicine and Medical Research 24(9): 1-11, 2017; Article no.JAMMR.36876 ISSN: 2456-8899 (Past name: British Journal of Medicine and Medical Research, Past ISSN: 2231-0614, NLM ID: 101570965) CARBA NP a Rapid and Reliable Test for Detection of Carbapenemases in Gram Negative Bacteria Saba Choudhary 1 , Bashir Ahmad Fomda 1* , Munazah Manzoor 1 , Gulnaz Bashir 1 , Asiya Khan 1 and Mir Yawar 1 1 Department of Microbiology, Sher-i-Kashmir Institute of Medical Sciences, Srinagar, Jammu and Kashmir, India. Authors’ contributions This work was carried out in collaboration between all authors. Author SC wrote the first draft of the manuscript, wrote the protocol and performed the experiments. Author BAF designed the study, wrote the protocol, supervised the experimental work and wrote the manuscript. Author GB helped in writing protocol. Author AK helped in performing the experiments. Author MM managed the literature searches. Author MY managed the analyses of the study, performed the statistical analysis and helped in writing the manuscript. All authors read and approved the final manuscript. Article Information DOI: 10.9734/JAMMR/2017/36876 Editor(s): (1) Kalpy Julien Coulibaly, Département Environnement et Santé (Intérim), Institut Pasteur de Côte d'Ivoire, Université Félix Houphouet-Boigny, Abidjan, Côte d’Ivoire. Reviewers: (1) Wagih Mommtaz Ghannam, Mansoura University, Egypt. (2) Magda Ramadan Abdelwadood, Ain Shams University, Egypt. Complete Peer review History: http://www.sciencedomain.org/review-history/22237 Received 21 st September 2017 Accepted 20 th October 2017 Published 11 th December 2017 ABSTRACT Both molecular and non-molecular tests currently employed for the detection of carbapenemases are costly, time-consuming and are poorly adapted to the clinical need for isolating patients rapidly to prevent health care associated outbreaks. The present study was thus conducted for rapid detection of carbapenemases in clinical isolates of Gram negative bacteria by Carba NP test. A total of 270 Gram-negative bacilli included were divided into three groups Group I (n=100): Gram negative bacteria isolated from routine samples. Group II (n=130): Imipenem resistant Gram negative bacteria. Group III (n=40): Twenty PCR confirmed for bla VIM and bla NDM1 and twenty PCR negative for bla VIM and bla NDM1 isolates. These isolates were subjected to Carba NP test. Antimicrobial susceptibility was performed by Kirby Bauer disk diffusion method. Out of 100 isolate (Group I), 32 were positive and out of 130 imipenem resistant (Group II), 112 isolates were positive for carbapenemase production by carba NP test. The sensitivity and specificity was 100% for Original Research Article