Development & Validation of HPLC method for simultaneous estimation of Drotaverine & Omeprazole Zaheer Shaikh 1 , Abdul Ahad 2 , Showkat Patel 3 and Maqdoom Farooqui 4* 1 Wockhardt Ltd, MIDC, Chilkalthana, Aurangabad (M.S.) India. 2 Post graduate and Research Centre, Maulana Azad College, Aurangabad (M.S), India. 3 Adarsh College, Omerga. Dist, Osmanabad (M.S), India 4* Dr.Rafiq Zakaria College for Women, Aurangabad (M.S), India Abstract : A simple and accurate method was developed for simultaneous estimation of Drotaverine & Omeprazole. The chromatography parameters includes stainless steel column Develosil ODS HG-5 RP C 18 , 5m, 15cmx4.6mm i.d and acetonitrile: potassium dihydrogen phosphate buffer (0.02M, pH 3.0) (55:45v/v) as mobile phase at a flow rate (1.0 ml/minute). The determinations were performed using UV-Vis detector set at 273 nm. The developed HPLC method showed specificity and selectivity with good precision and accuracy, which makes it very suitable for quantification of Drotaverine & Omeprazole. Key words: HPLC, simultaneous estimation, Drotaverine ,Omeprazole. Introduction Pharmaceutical analysis [1-3] plays a very significant and vital role in the evaluation of pharmaceutical formulations and bulk drugs with respect to the assurance and quality control [1-3]. The improvements in analytical method developments and instruments have reduced the time and cost of analysis and enhanced precision and accuracy.[4]Drotaverine (DRO) is has IUPAC name 1-[(3,4-[diethoxyphenyl) methylene]-6,7- diethoxy-1,2,3,4-tetrahydroisoquinolene and used as an antispasmodic and smooth-muscle relaxant in pain related with biliary colic, and postsurgical spasms. [5,6]. It affects by inhibiting phosphodiesterase enzyme IV which is particular utilized for smooth muscles [7,8]. Omeprazole drug is a used as an antiulcer drug and also used against other acid-related diseases [9]. Omeprazole acts as the proton pump inhibitor which in the acidic PH of the stomach, reacts with a cysteine group in H+/K+-ATPase, thus impede the ability of the parietal cells to generate gastric acid (Tripathi, 2008) [10] . The present work describes a new precise, simple, rapid and accurate method for simultaneous estimation of drotaverine hydrochloride and omeprazole in combined dosage form. Maqdoom Farooqui et al /International Journal of ChemTech Research, 2020,13(1): 01-10. http://dx.doi.org/10.20902/IJCTR.2019.130101 International Journal of ChemTech Research CODEN (USA): IJCRGG, ISSN: 0974-4290, ISSN(Online):2455-9555 Vol.13 No.01, pp 01-10, 2020