Supporting information Taking multicalixarenes into the nanoworld: First third- generation calixarene dendrimer Ruth Lalor, a A. Patrick Gunning ,b Victor J. Morris b and Susan E. Matthews* a General Experimental Details All chemicals were purchased from Sigma-Aldrich Ltd. (Aldrich, Sigma and Fluka/Riedel de Haën brands), Lancaster or Acrôs Organics, and unless specified, were used without further purification. Deuterated solvents for NMR use were purchased from Cambridge Isotope Laboratories, Inc. or Apollo Scientific. Analytical thin layer chromatography (TLC) was performed using Merck Kieselgel 60 F 254 silica gel plates. Column chromatography was run using silica gel 60 (70-230 mesh ASTM). Visualisation was by UV light (254 nm). NMR spectra were recorded at 293 K, unless otherwise stated, using a 400 MHz Varian Unity Plus Spectrometer, operating at 399.92 MHz or 399.96 MHz (proton), 100.56 MHz or 100.57 MHz (carbon) and 376.24 MHz (fluorine), or a 300 MHz Gemini 2000 spectrometer operating at 300.05 MHz (proton) and 75.45 MHz (carbon). Shifts are referenced relative to the internal reference standard, tetramethylsilane (TMS), with chemical shifts expressed in ppm or δ downfield from the standard and coupling constants (J) expressed in Hz. NMR data were processed using MestReC software. MALDI-TOF mass spectra were recorded by the EPSRC National Mass Spectrometry Service, Swansea, or using a Kratos Axima CFR MALDI Mass Spectrometer. ESI mass spectra were recorded on a Shimadzu LC-MS 2010EV Spectrometer, with HPLC grade CH 3 OH, water or CH 3 CN as carrier solvents. Melting points were measured using an Electrothermal Mel-temp® melting point apparatus and are reported uncorrected. Infrared spectra were recorded using an Avatar 360 FT-IR spectrometer. Supplementary Material (ESI) for Chemical Communications This journal is (c) The Royal Society of Chemistry 2010