56 Akta Agrosia. 2019. 22(2):56-62 Cited this as : R.A. Sari, R. Herawati, and C. Herison Induction and growth of endosperm callus of Rimau Gerga Lebong (RGL) citrus on several media composition. ISSN: 1410-3354 / e-ISSN:2615-7136 ARTICLE INFO Keywords: RGL citrus Calli Endosperm Kinetin BAP Article history: Received: November 11, 2018 Accepted: December 19, 2019 Published: December 29, 2019 *Corresponding author: E-mail: reny.herawati@unib.ac.id ABSTRACT Rimau Gerga Lebong (RGL) variety is one of the main orange fruit com- modities in Lebong Regency of Bengkulu Province, which has a competi- tive advantage and has good market potential. However, high number of seed characteristic makes this orange fruit becomes less popular. Triploid genotype formation through endosperm culture in vitro is an alternative solution to develop seedless orange fruit. The objective of this study was to determine the best composition medium for callus induction of endo- sperm of RGL orange seeds as the foremost step of in vitro triploid plant development. The research was conducted from August 2017 until De- cember 2018 at the Plant Tissue Culture Laboratory, Department of Agronomy, Faculty of Agriculture, University of Bengkulu. The experi- ment was arranged in a completely randomized design, with eight treat- ment combinations i.e. G1 (MT + 5 ppm BAP + 2 ppm 2,4-D), G2 (MT + 5 ppm BAP + 2 ppm 2,4-D + 500 ppm CH), G3 (MT + 5 ppm BAP + 2 ppm 2,4-D + 500 ppm + 0.5 ppm Kinetin), G4 (MT + 5 ppm BAP + 2 ppm 2,4- D + 500 ppm CH + 0.5 ppm to 500 ppm Kinetin + ME) , G5 (MS + 5 ppm BAP + 2 ppm 2,4-D), G6 (MS + 5 ppm BAP + 2 ppm 2,4-D + 500 ppm CH), G7 (MS + 5 ppm BAP + 2 ppm 2,4- D + 500 ppm CH + 0.5 ppm Ki- netin), G8 (MS + 5 ppm BAP + 2 ppm 2,4-D + 500 ppm CH + 0.5 ppm to 500 ppm Kinetin + ME), with three replications. The experimental unit was five culture bottles containing three explants per bottle. Observations were conducted on days to callus formation, rate of callus formation, callus weight, callus diameter, callus color and callus texture. The results showed that media of MS + 5 ppm BAP + 2 ppm 2,4-D + 500 ppm CH was the best media for callus induction, and MT + 5 ppm BAP + 2 ppm 2,4-D + 500 ppm CH was the best media for callus development. Akta Agrosia Induction and Growth of Endosperm Callus of Rimau Gerga Le- bong (RGL) Citrus on Several Media Composition Rossi Anandayu Sari, Reny Herawati*, Catur Herison Department of Crop Production, Faculty of Agriculture, University of Bengkulu WR. Supratman St, Bengkulu 38371, Indonesia INTRODUCTION Citrus is horticultural commodities that serve as a source of nutrition, a source of income, and a source of foreign exchange. The contribution of citrus agro-industry in increasing revenue will foster new citrus development centres. The availability of superior varieties, both quality and productivity by consumer needs, is an absolute that must be met in the global free market. Achieve a balance between demand and supply; national citrus production needs to be increased continuously (Karsinah et al., 2002). Production of tangerines in Indonesia in the last five years fluctuated, in 2012 the production of tangerines amounted to 1,498,396 tons in 2013 of 1,548,401 tons in 2014 amounted to 1,785,264 tons in 2015 reached 1,744,339 tons and in 2016 reached