256 SMYTE Vol. 42 PPA-n, the antioxidant activity is chain-length-dependent, but this correlation is not clear and differs in the two series. ioo % 50 100 % 50 I I I i - I I I I I ! 8 12 16 n Fig. 1. Dependence of inhibition of erythrocyte ghost oxidation on alkyl chain length of compounds PPE-n (top) and PPA-n (bottom). <~- 30 rain; A - 60 min. 0.8 A535 0.6 0./, 0.2 I I 20 l.O rain Fig. 2. Time-dependent liposome oxidation in the pre- sence of compounds PPA-n. O - control; O - n = 8; D-n= 12;& -n= 16. [1] BARTOSZ G.: The SecondFace of Oxygen, p. 94-114. (In Polish) PWN, Warsaw 1995. [2] HALLIWELL B., GUTTER1DGE J.M.C.: Free Radicals m Biology andMedicme. Clarendon Press, Oxford 1989. [3] W1TEKS., OSWII~CIMSKA M., LACHOWICZ T.M., BALAKUSZEW A., PRZESTALSKI S., KUCZERA J., SARAPUK J., KLESZCZYIqSKA H., GABRIELSKA J., HLADYSZOWSK1J.,TRELA Z., KRALT., PODOLAK N.: Folia Microbiol. 39, 559-560 (1994). [4] DEVINSK~" F., MASAI~OVA L., LAt~KO I., MLYNARg:iK D.: J.Biopharm.Sci. 2, 1-10 (1991). [5] LINSTEDT M., ALLENMARK S., THOMPSON R.A., EDEBOL.: Antimicrob.Agents Chemother. 34, 1949-1954 (1990). [6] LACHOWICZT.M., WITEK S., LUCZY~qSKI J., WITKOWSKAR., BALAKUSZEW A., KLESZCZYIqSKA H., KRAL T., KUCZERA J., PRZESTALSKI S.: Folia MicrobioL 41, 102-105 (1996). [7] BUEGE J.A., AUST S.D: Methods Enzymol. 52C, 302-310 (1978). [8] PORI~BSKA-BUDNY M., SAKINA N.L., STI~PIElq K.B., DONTSOV A, E., WILCZOK T.: Biochim. Biophys.A cta I I 16, 11- 16 (1992). Expression of Genes Coding for ADP/ATP Translocator in Various Yeasts I. ZEMAN a, J. KOLAROV a,b aDepartment of Biochemistry, Faculty of Science, Comenius University, 842 15 Bratislava bCancer Research Institute, Slovak Academy of Sciences, 812 32 Bratislava, Slovakia From a number of yeasts screened in our laboratory, Saccharomyces cerevisiae and Brettanomyces anomalus were able to grow under strict anaerobiosis. The inability of eukaryotic cells to grow under strict anaerobiosis can result from: (/) low fermentative capacity; (ii) oxygen-requiring metabolic pathways; (iii) disturbed redox state; (iv) demand for functional respiration chain. Another cellular function required for anaerobiosis is presumably the mitochondrial adenine nu- cleotide translocation. Intramitochondrial ATP is necessary for a cell both under aerobic and anaerobic conditions [ l, 2]. The mitochondrial ADP/ATP translocator in S. cerevisiae is essential for anaerobic growth [3] and its specific inhibition with bongkrekic acid arrested anaerobic growth of S. cerevisiae as well of B. anomalus. Three isogenes encoding the ADP/ATP carrier in S. cerevisiae are differentially expressed and the common effector molecule that plays a pivotal role in the regulation of their expression is oxygen. The AAC1 and AAC2 genes are expressed preferentially in the presence of oxygen, while the third gene, AAC3,